製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to Taf4
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Drosophila melanogaster
  • 免疫原

    Fusion protein: MNTSQTAAGN RITFTSQPLP NGTISIAGNP GAVISTAQLP NTTTIKTIQA GIGGQHQGLQ QVHHVQQQQQ SQQQQQQQQQ TQSAGQPLLN SMLPAGVVVG , corresponding to amino acids 695-794 of Fruit fly (Drosophila melanogaster) Taf4

  • 特記事項

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • Concentration information loading...
  • 一次抗体 備考
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 110 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 関連性
    Taf4 makes part of TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It potentiates transcriptional activation by the AF-2S of retinoic acid, vitamin D3 and thyroid hormone.
  • 細胞内局在
    Nuclear
  • 参照データベース
    • 別名
      • TAF2C antibody
      • TAF2C1 antibody
      • TAF4A antibody
      • TAFII 130 antibody
      • TAFII 135 antibody
      • TBP associated factor 4 antibody
      see all

    画像

    • All lanes : Anti-Taf4 antibody (ab52846) at 1/1000 dilution

      Lane 1 : Total protein extract from E coli with ~50ng to 100ng of an irrelevant antigen
      Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 110 kDa

    プロトコール

    参考文献

    ab52846 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Western blot
    Sample
    Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Drosophila melanogaster cell line S2)
    Loading amount
    20 µg
    Specification
    Drosophila melanogaster cell line S2
    Gel Running Conditions
    Non-reduced Denaturing (8% acrylamide)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    投稿 Jul 15 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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