The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:12500
Use a concentration of 2.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 95 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. Recruits RNA polymerase I to the rRNA gene promoter via interaction with RRN3.
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.