Anti-BTAF1 抗体 [BTA3D61] (ab50990)
Key features and details
- Mouse monoclonal [BTA3D61] to BTAF1
- Suitable for: WB, IP, ICC, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-BTAF1 antibody [BTA3D61]
BTAF1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [BTA3D61] to BTAF1 -
由来種
Mouse -
アプリケーション
適用あり: WB, IP, ICC, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant full length protein corresponding to BTAF1.
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ポジティブ・コントロール
- WB: HEK293, NIH3T3 and F2408 whole cell lysates. ICC/IF and FACS: HeLa cells. IP: RIPA lysate of HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
BTA3D61 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50990の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa).
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IP |
Use at an assay dependent concentration.
Use at 5µg/0.5mg of lysate. |
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ICC |
1/10.
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Flow Cyt |
1/10.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/50. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa). |
IP
Use at an assay dependent concentration. Use at 5µg/0.5mg of lysate. |
ICC
1/10. |
Flow Cyt
1/10. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner. -
配列類似性
Belongs to the SNF2/RAD54 helicase family.
Contains 8 HEAT repeats.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9044 Human
- Entrez Gene: 107182 Mouse
- Entrez Gene: 368042 Rat
- Omim: 605191 Human
- SwissProt: O14981 Human
- Unigene: 500526 Human
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別名
- ATP dependent helicase BTAF1 antibody
- ATP-dependent helicase BTAF1 antibody
- B-TFIID transcription factor associated 170 kDa subunit antibody
see all
画像
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Anti-BTAF1 antibody [BTA3D61] (ab50990) at 1/50 dilution + HEK293 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 207 kDa
Observed band size: 207 kDa -
HeLa cells stained with ab50990 at 1/10 dilution followed by Alexa Fluor 488 Goat Anti-Mouse IgG at 1/200.
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Ab50990 used at 5µg/0.5mg RIPA lysate of HeLa cells. The immunoprecipitate was subjected to Western blotting using ab50990 at 1/100. The ECL technique was used, and the secondary antibody was Mouse IgG at 1/2500.
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Cells labelled with ab50990 at 1/10 dilution followed by Alexa Fluor 488 Goat Anti-Mouse IgG at 1/400 dilution.
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Anti-BTAF1 antibody [BTA3D61] (ab50990) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 207 kDa
Observed band size: 207 kDa -
Anti-BTAF1 antibody [BTA3D61] (ab50990) at 1/50 dilution + F2408 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 207 kDa
Observed band size: 207 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab50990 は論文での使用が確認できていません。