製品の概要

  • 製品名
    Anti-TAF172 antibody
    TAF172 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TAF172
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to a region between residues 75 and 125 of human TAF172 (NP_003963.1).

  • ポジティブ･コントロール
    • Whole cell lysates from HeLa and 293T cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner.
  • 配列類似性
    Belongs to the SNF2/RAD54 helicase family.
    Contains 8 HEAT repeats.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
    • 別名
      • ATP dependent helicase BTAF1 antibody
      • ATP-dependent helicase BTAF1 antibody
      • B-TFIID transcription factor associated 170 kDa subunit antibody
      • B-TFIID transcription factor associated, 170kDa (Mot1 homolog, S. cerevisiae) antibody
      • B-TFIID transcription factor-associated 170 kDa subunit antibody
      • BTAF 1 RNA polymerase II antibody
      • BTAF1 antibody
      • BTAF1 RNA polymerase II B TFIID transcription factor associated 170kDa antibody
      • BTAF1_HUMAN antibody
      • MOT 1 antibody
      • MOT1 antibody
      • MOT1 Yeast homolog of antibody
      • TAF 172 antibody
      • TAF II 170 antibody
      • TAF(II)170 antibody
      • TAF-172 antibody
      • TAFII170 antibody
      • TATA binding protein associated factor 172 antibody
      • TATA-binding protein-associated factor 172 antibody
      • TBP associated factor 172 antibody
      • TBP-associated factor 172 antibody
      see all

    画像

    • Western blot - TAF172 antibody (ab72285)
      Western blot - TAF172 antibody (ab72285)
      All lanes : Anti-TAF172 antibody (ab72285) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size: 207 kDa
      Observed band size: 207 kDa
      Additional bands at: 170 kDa, 85 kDa. We are unsure as to the identity of these extra bands.

    • Immunoprecipitation - TAF172 antibody (ab72285)
      Immunoprecipitation - TAF172 antibody (ab72285)
      Detection of Human TAF172 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab72285 at 3µg/mg lysate for IP and at 1µg/ml for subsequent WB.

    参考文献

    ab72285 has not yet been referenced specifically in any publications.

    ab72285 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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