製品の概要
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製品名Anti-TAF172 antibody
TAF172 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to TAF172
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アプリケーション適用あり: WB, IPmore details
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種交差性交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey
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免疫原
Synthetic peptide corresponding to a region between residues 75 and 125 of human TAF172 (NP_003963.1).
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ポジティブ･コントロール
- Whole cell lysates from HeLa and 293T cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab72285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner.
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配列類似性Belongs to the SNF2/RAD54 helicase family.
Contains 8 HEAT repeats.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 9044 Human
- Omim: 605191 Human
- SwissProt: O14981 Human
- Unigene: 500526 Human
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別名
- ATP dependent helicase BTAF1 antibody
- ATP-dependent helicase BTAF1 antibody
- B-TFIID transcription factor associated 170 kDa subunit antibody
see all
Anti-TAF172 antibody 画像
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Western blot - TAF172 antibody (ab72285)All lanes : Anti-TAF172 antibody (ab72285) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 207 kDa
Observed band size : 207 kDa
Additional bands at : 170 kDa,85 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - TAF172 antibody (ab72285)Detection of Human TAF172 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab72285 at 3µg/mg lysate for IP and at 1µg/ml for subsequent WB.
Anti-TAF172 antibody (ab72285) 使用論文
ab72285 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"