The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報IHC-P: 1/100 - 1/500.
Antigen exposure is recommended.
Antigen exposure with citrate buffer will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
組織特異性Ubiquitous. Observed in all fetal and adult tissues.
関連疾患Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
配列類似性Belongs to the RRM TET family. Contains 1 RanBP2-type zinc finger. Contains 1 RRM (RNA recognition motif) domain.
翻訳後修飾Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity. Phosphorylated upon DNA damage, probably by ATM or ATR.
細胞内局在Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
ab84369 at 1/250, staining human TAF15 in a section of human stomach adenocarcinoma by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection: DAB staining using anti-rabbit antibody at 1/100.
Anti-TAF15 antibody (ab84369) 使用論文
has not yet been referenced specifically in any publications.