The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
Use a concentration of 1 - 5 µg/ml.
機能RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
組織特異性Ubiquitous. Observed in all fetal and adult tissues.
関連疾患Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
配列類似性Belongs to the RRM TET family. Contains 1 RanBP2-type zinc finger. Contains 1 RRM (RNA recognition motif) domain.
翻訳後修飾Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity. Phosphorylated upon DNA damage, probably by ATM or ATR.
細胞内局在Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.