Anti-TACD2 抗体 (ab65006)

製品の概要

  • 製品名Anti-TACD2 antibody
    TACD2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TACD2
  • 特異性This antibody detects endogenous levels of total TACD2 protein.
  • アプリケーション適用あり: IHC-P, WB, ELISA, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Pig
  • 免疫原

    A synthetic peptide derived from internal region of human TACD2

  • ポジティブ・コントロール
    • Extracts from A549 and NIH-3T3 cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab65006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ELISA 1/5000.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能May function as a growth factor receptor.
  • 組織特異性Placenta, pancreatic carcinoma cell lines.
  • 関連疾患Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment.
  • 配列類似性Belongs to the EPCAM family.
    Contains 1 thyroglobulin type-1 domain.
  • 翻訳後修飾The N-terminus is blocked.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell surface glycoprotein Trop 2 antibody
    • Cell surface glycoprotein Trop-2 antibody
    • Cell surface glycoprotein Trop2 antibody
    • Epithelial glycoprotein 1 antibody
    • GA733 1 antibody
    • GA7331 antibody
    • M1S 1 antibody
    • M1S1 antibody
    • Membrane component chromosome 1 surface marker 1 antibody
    • Pancreatic carcinoma marker protein GA733 1 antibody
    • Pancreatic carcinoma marker protein GA733-1 antibody
    • Pancreatic carcinoma marker protein GA7331 antibody
    • TACD 2 antibody
    • TACD2_HUMAN antibody
    • TACSTD 2 antibody
    • Tacstd2 antibody
    • Trop 2 antibody
    • Trop2 antibody
    • Tumor associated calcium signal transducer 2 precursor antibody
    • Tumor-associated calcium signal transducer 2 antibody
    see all

Anti-TACD2 antibody 画像

  • All lanes : Anti-TACD2 antibody (ab65006) at 1/500 dilution

    Lane 1 : Extracts from A549 cells
    Lane 2 : Extracts from NIH-3T3 cells
    Lane 3 : Extracts from NIH-3T3 cells with immunising peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 37 kDa
    Observed band size : 37 kDa
  • IHC image of ab65006 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65006, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-TACD2 antibody (ab65006) 使用論文

ab65006 has not yet been referenced specifically in any publications.

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Vielen Dank für Ihre Anrufe.

Wie versprochen habe ich alle unsere Langerin- bzw. TACD2-Antikörper danach geprüft, ob sie schon einmal mit Schwein getestet wurden - was leider bei keinem der Fall ist.

Daher habe ich di...

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I am sorry about all of the problems you have been having with these antibodies.

It would greatly be appreciated if you could send me some of the images that you are getting using these antibodies and...

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Thank you for contacting us again. And thanks to the customer for trying the suggestions and optimising the protocol. It is regrettable the results have not been successful. To be honest, I have no clue why the antibody does not work for the positive c...

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Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products. We would be pleased to arrange a replacement product free of charge with ab7910. I am forwarding these details on to our Distributor’s T...

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Thank you for kindly confirming these details.  To me the results that you obtained from ab65006 for the urine samples seem to be specific: According to the literature the tumor-associated calcium signal transducer 2 TACD2 is glycosylated which cou...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this TACD2 antibody. The details you have kindly provided will enable us to investigate this c...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (Prostate)
Specification Prostate
Fixative Methanol
Permeabilization Yes - Trixton X-100
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

投稿 Jun 17 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Prostate)
Loading amount 25 µg
Specification Prostate
Gel Running Conditions Non-reduced Denaturing (10%gel)
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

投稿 Apr 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"