Anti-T7 tag® 抗体 (HRP) (ab21477)
製品の概要
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製品名Anti-T7 tag® antibody (HRP)
T7 tag® 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to T7 tag® (HRP)
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由来種Rabbit
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標識HRP
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特異性Ab21477 recognises T7 tag.
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アプリケーション適用あり: ELISA, WB, ICCmore details
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種交差性May react with Bordetella phage BPP-1, Yersinia pestis phage phiA1122, Bacteriophage T7, based on sequence homology. Not yet tested in other species.
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免疫原
Synthetic peptide:
MASMTGGQQMG
conjugated to KLH, corresponding to amino acids 1-11 of T7 tag -
特記事項T7-TAG® is a trademark of EMD Biosciences, Inc.
法規制情報
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C.
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バッファーPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.2% BSA, 50mM HEPES, 100mM Sodium chloride, pH 7.1 -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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アプリケーション
Our Abpromise guarantee covers the use of ab21477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | Use a concentration of 0.01 - 0.1 µg/ml. | |
| WB | Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 42 kDa. | |
| ICC | Use a concentration of 0.5 - 2 µg/ml. |
ターゲット情報
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関連性The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
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別名
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
see all
参考文献
This product has been referenced in:
- Li Y & Smolke CD Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. Nat Commun 7:12137 (2016). WB . Read more (PubMed: 27378283) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"