製品の概要

  • 製品名Anti-T7 tag® antibody - ChIP Grade
    T7 tag® 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to T7 tag® - ChIP Grade
  • 特異性This antibody was verified by ELISA against peptide conjugated to BSA(MASMTGGQQMG/BSA). Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • アプリケーション適用あり: ELISA, IP, WB, ICC, ChIPmore details
  • 免疫原

    Synthetic peptide:

    MASMTGGQQMG

    (T7) conjugated to KLH.

  • 特記事項T7-TAG® is a trademark of EMD Biosciences, Inc.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was affinity purified using the peptide immobilized on solid support..
  • ポリ/モノポリクローナル
  • アイソタイプunknown
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution. Use at a dilution of: Coating 1/100 - 1/500, Primary 1/1000 - 1/30,000.
IP Use at an assay dependent dilution.
WB 1/1000 - 1/30000.
ICC 1/200 - 1/2000.
ChIP Use at an assay dependent dilution. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.

ターゲット情報

  • 関連性The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • 別名
    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

Anti-T7 tag® antibody - ChIP Grade 画像

  • A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).

Anti-T7 tag® antibody - ChIP Grade (ab9138) 使用論文

This product has been referenced in:

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T cells)
Loading amount 15 µg
Specification 293T cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Feb 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (293T cells)
Total protein in input 1e+006 cells
Specification 293T cells
Immuno-precipitation step Protein A/G
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投稿 Feb 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"