製品の概要

  • 製品名Anti-T-bet / Tbx21 antibody
    T-bet / Tbx21 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to T-bet / Tbx21
  • アプリケーション適用あり: IP, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human T-bet/ Tbx21.

    (Peptide available as ab69655.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Jurkat Whole Cell, Jurkat Nuclear, Jurkat Whole Cell - Staurosporine Treated (24hr, 500nM), E14Tg2a Whole Cell

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).

ターゲット情報

  • 機能Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
  • 組織特異性T-cell specific.
  • 関連疾患Genetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities.
  • 配列類似性Contains 1 T-box DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • T bet antibody
    • T box 21 antibody
    • T box expressed in T cells antibody
    • T box protein 21 antibody
    • T box transcription factor TBX21 antibody
    • T cell specific T box transcription factor antibody
    • T cell specific T box transcription factor T bet antibody
    • T PET antibody
    • T-box protein 21 antibody
    • T-box transcription factor TBX21 antibody
    • T-cell-specific T-box transcription factor T-bet antibody
    • TBET antibody
    • TBLYM antibody
    • TBX 21 antibody
    • Tbx21 antibody
    • TBX21_HUMAN antibody
    • TPET antibody
    • Transcription factor TBLYM antibody
    see all

Anti-T-bet / Tbx21 antibody 画像

  • T-bet / Tbx21 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab53174.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 53kDa; T-bet / Tbx21

  • All lanes : Anti-T-bet / Tbx21 antibody (ab53174) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)
    Lane 3 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

Anti-T-bet / Tbx21 antibody (ab53174) 使用論文

ab53174 has not yet been referenced specifically in any publications.

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