製品の概要

  • 製品名Anti-SYT2 antibody
    SYT2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to SYT2
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Rhesus monkey, Opossum
  • 免疫原

    Recombinant fragment with tag: KIHLMQNGKR LKKKKTTVKK KTLNPYFNES FSFEIPFEQI QKVQVVVTVL DYDKLGKNEA IGKIFVGSNA TGTELRHWSD MLANPRRPIA QWHSLKPEEE VDALLGKN, corresponding to amino acids 311-419 of Human SYT2

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab60716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: Use at an assay dependent dilution. The detection limit for recombinant tagged SYT2 is approximately 0.1ng/ml as a capture antibody.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 47 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
    • 配列類似性Belongs to the synaptotagmin family.
      Contains 2 C2 domains.
    • ドメインThe first C2 domain mediates Ca(2+)-dependent phospholipid binding.
      The second C2 domain mediates interaction with Stonin 2.
    • 細胞内局在Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Synaptic vesicles and chromaffin granules.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Synaptotagmin 2 antibody
      • Synaptotagmin II antibody
      • Synaptotagmin-2 antibody
      • Synaptotagmin2 antibody
      • SynaptotagminII antibody
      • SYT 2 antibody
      • Syt II antibody
      • Syt2 antibody
      • SYT2_HUMAN antibody
      • SytII antibody
      see all

    Anti-SYT2 antibody 画像

    • Anti-SYT2 antibody (ab60716) at 1 µg/ml + HepG2 cell lysate at 25 µg

      Predicted band size : 47 kDa
      Observed band size : 44 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

    Anti-SYT2 antibody (ab60716) 使用論文

    ab60716 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Recombinant protein (partial recombinant protein)
    Loading amount 0.02 µg
    Specification partial recombinant protein
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Mr. Benjamin Wilhelm

    Verified customer

    投稿 Apr 18 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - other (Brain)
    Loading amount 10 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 18 2012

    DISCOUNT CODE: x

    Ablaufdatum: ddmm2012
    Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab60716 in Maus, Ratte und Humanem Gewebe zu testen, annehmen möchten. Wie besprochen, können Sie den Gutscheincode zum Kauf eines w...

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    Vielen Dank für Ihre Rückmeldung.
    Die Auswahl des Immunogens basiert oftmals auf Vorhersagen, wo in einem Protein sich geeignete Immunogenpeptidsequenzen befinden können. Oder es wird der Proteinteil zugrunde gelegt, gegen den ein Antikörper produ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"