Anti-SYT2 抗体 (ab113545)
Key features and details
- Rabbit polyclonal to SYT2
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SYT2 antibody
SYT2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SYT2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Dog, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Common marmoset, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in the following Mouse tissue lysates: Brain; Spinal Cord; Cortex; Hippocampus; Cerebellum; Forebrain; Substantia Nigra. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal brain.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113545の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (6) |
Use a concentration of 0.5 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).
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特記事項 |
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IHC-P
Use a concentration of 0.5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa). |
ターゲット情報
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機能
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. -
配列類似性
Belongs to the synaptotagmin family.
Contains 2 C2 domains. -
ドメイン
The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with Stonin 2. -
細胞内局在
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Synaptic vesicles and chromaffin granules. - Information by UniProt
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参照データベース
- Entrez Gene: 127833 Human
- Entrez Gene: 20980 Mouse
- Entrez Gene: 24805 Rat
- Omim: 600104 Human
- SwissProt: Q8N9I0 Human
- SwissProt: P46097 Mouse
- SwissProt: P29101 Rat
- Unigene: 25422 Human
see all -
別名
- Synaptotagmin 2 antibody
- Synaptotagmin II antibody
- Synaptotagmin-2 antibody
see all
画像
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IHC-P image of SYT2 staining on sections from rat thalamic nucleus (upper image) and the CA3 region of the rat hippocampus (lower image) using ab113545 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours 10 minutes at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
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IHC-P image of SYT2 staining on sections from mouse sagittal hippocampus (upper image) and dentate nucleus of the mouse cerebellum (lower image) using ab113545 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
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IHC-P image of SYT2 staining on Marmoset cerebellum sections using ab113545(1:7000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:7000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
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All lanes : Anti-SYT2 antibody (ab113545) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lane 5 : Cerebellum Mouse Tissue Lysate
Lane 6 : Forebrain (Mouse) Tissue Lysate
Lane 7 : Mouse Substantia Nigra Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
The predicted molecular weight of SYT2 is 47 kDa (SwissProt), however we expect to observe a banding pattern around 70 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
IHC image of SYT2 staining in Mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113545, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab113545 は 5 報の論文で使用されています。
- Naef V et al. Converging Role for REEP1/SPG31 in Oxidative Stress. Int J Mol Sci 24:N/A (2023). ace2 ; Human . PubMed: 36834939
- Lai Y et al. Inhibition of calcium-triggered secretion by hydrocarbon-stapled peptides. Nature 603:949-956 (2022). PubMed: 35322233
- Naef V et al. Efficient Neuroprotective Rescue of Sacsin-Related Disease Phenotypes in Zebrafish. Int J Mol Sci 22:N/A (2021). PubMed: 34445111
- Iacomino M et al. Distal motor neuropathy associated with novel EMILIN1 mutation. Neurobiol Dis 137:104757 (2020). PubMed: 31978608
- Connan C et al. Botulinum neurotoxin type B uses a distinct entry pathway mediated by CDC42 into intestinal cells versus neuronal cells. Cell Microbiol 19:N/A (2017). PubMed: 28296078