製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to SYT2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human
  • 免疫原

    Synthetic peptide:

    C-KPEEEVDALLGKNK

    , corresponding to C terminal amino acids 406-419 of Human SYT2 (NP_796376.2).

  • ポジティブ・コントロール
    • Mouse lung lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab109987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).

ターゲット情報

  • 機能
    May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
  • 配列類似性
    Belongs to the synaptotagmin family.
    Contains 2 C2 domains.
  • ドメイン
    The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
    The second C2 domain mediates interaction with Stonin 2.
  • 細胞内局在
    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Synaptic vesicles and chromaffin granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Synaptotagmin 2 antibody
    • Synaptotagmin II antibody
    • Synaptotagmin-2 antibody
    • Synaptotagmin2 antibody
    • SynaptotagminII antibody
    • SYT 2 antibody
    • Syt II antibody
    • Syt2 antibody
    • SYT2_HUMAN antibody
    • SytII antibody
    see all

画像

  • Anti-SYT2 antibody (ab109987) at 0.1 µg/ml + Mouse lung lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

プロトコール

参考文献

ab109987 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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