製品の概要

  • 製品名Anti-Syntrophin antibody [1351]
  • 製品の詳細
    Mouse monoclonal [1351] to Syntrophin
  • 特異性ab11425 is known to be reactive with the alpha 1, beta 1 and beta 2 subunits of syntrophin.
  • アプリケーション適用あり: ICC/IF, IP, WB, IHC-Fr, ICC, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Dog, Human, Xenopus laevis, Amphibians, Xenopus tropicalis
    交差が予測される動物種: Fish
  • 免疫原

    Other Immunogen Type corresponding to Syntrophin. Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.

  • エピトープab11425 is directed against an epitope within the PDZ domain of syntrophin.
  • ポジティブ・コントロール
    • In Western Blot, this antibody gave a positive signal in human, mouse and rat skeletal muscle tissue lysates.
  • 特記事項

    ab11425 is seen as the "gold standard" for syntrophin assessment.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 0.2 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 54 kDa).
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex. Plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Binds to phosphatidylinositol 4,5-bisphosphate.
  • 組織特異性High expression in skeletal muscle and heart. Low expression in brain, pancreas, liver, kidney and lung. Not detected in placenta.
  • 関連疾患Long QT syndrome 12 (LQT12) [MIM:612955]: A heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • 配列類似性Belongs to the syntrophin family.
    Contains 1 PDZ (DHR) domain.
    Contains 2 PH domains.
    Contains 1 SU (syntrophin unique) domain.
  • ドメインThe PH 1 domain mediates the oligomerization in a calcium dependent manner, and the association with the phosphatidylinositol 4,5-bisphosphate.
    The PDZ domain binds to the last three or four amino acids of ion channels and receptor proteins. The association with dystrophin or related proteins probably leaves the PDZ domain available to recruit proteins to the membrane.
    The SU domain binds calmodulin in a calcium-dependent manner.
  • 翻訳後修飾Phosphorylated by CaM-kinase II. Phosphorylation may inhibit the interaction with DMD.
  • 細胞内局在Cell membrane > sarcolemma. Cell junction. Cytoplasm > cytoskeleton. In skeletal muscle, it localizes at the cytoplasmic side of the sarcolemmal membrane and at neuromuscular junctions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 59 kDa dystrophin-associated protein A1 acidic component 1 antibody
    • Alpha-1-syntrophin antibody
    • Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody
    • SNT1 antibody
    • SNT2 antibody
    • SNT3 antibody
    • Snta1 antibody
    • SNTA1_HUMAN antibody
    • SNTB1 antibody
    • SNTB2 antibody
    • Syntrophin alpha 1 antibody
    • Syntrophin beta 1 antibody
    • Syntrophin beta 2 antibody
    • Syntrophin-1 antibody
    • TACIP1 antibody
    see all

Anti-Syntrophin antibody [1351] 画像

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Syntrophin (green) with ab11425 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of A2058 cells labeling Syntrophin (green) with ab11425 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of 293 cells labeling Syntrophin (green) with ab11425 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Overlay histogram showing SH-SY5Y cells stained with ab11425 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11425, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • All lanes : Anti-Syntrophin antibody [1351] (ab11425) at 5 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Rat) Tissue Lysate
    Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa,42 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • Syntrophin was immunoprecipitated using 0.5mg Mouse Skeletal Muscle tissue lysate, 5µg of Mouse monoclonal to Syntrophin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Skeletal Muscle tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab11425.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 58kDa; Syntrophin

Anti-Syntrophin antibody [1351] (ab11425) 使用論文

This product has been referenced in:
  • Kountz TS  et al. Endogenous N-terminal Domain Cleavage Modulates a1D-Adrenergic Receptor Pharmacodynamics. J Biol Chem 291:18210-21 (2016). Read more (PubMed: 27382054) »
  • Lundby A  et al. Annotation of loci from genome-wide association studies using tissue-specific quantitative interaction proteomics. Nat Methods 11:868-74 (2014). Read more (PubMed: 24952909) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (spinal cord membranous/cytosolic protein extract)
Loading amount 20 µg
Specification spinal cord membranous/cytosolic protein extract
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Jun 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"