製品の概要

  • 製品名Anti-Syntrophin alpha 1 antibody
    Syntrophin alpha 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Syntrophin alpha 1
  • 特異性This antibody recognizes alpha 1 syntrophin (60 kDa) but does not react with beta 1 and beta 2 syntrophins.
  • アプリケーション適用あり: Flow Cyt, IHC-Fr, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Rabbit, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 191-206 of Mouse Syntrophin alpha 1.

  • 特記事項For continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/1000.
ICC/IF 1/200.
WB 1/2000. Predicted molecular weight: 60 kDa.

ターゲット情報

  • 機能Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex. Plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Binds to phosphatidylinositol 4,5-biphosphate.
  • 組織特異性High expression in skeletal muscle and heart. Low expression in brain, pancreas, liver, kidney and lung. Not detected in placenta.
  • 関連疾患Defects in SNTA1 are the cause of long QT syndrome type 12 (LQT12) [MIM:612955]. A heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy.
  • 配列類似性Belongs to the syntrophin family.
    Contains 1 PDZ (DHR) domain.
    Contains 2 PH domains.
    Contains 1 SU (syntrophin unique) domain.
  • ドメインThe PH 1 domain mediates the oligomerization in a calcium dependent manner, and the association with the phosphatidylinositol 4,5-biphosphate.
    The PDZ domain binds to the last three or four amino acids of ion channels and receptor proteins. The association with dystrophin or related proteins probably leaves the PDZ domain available to recruit proteins to the membrane.
    The SU domain binds calmodulin in a calcium-dependent manner.
  • 翻訳後修飾Phosphorylated by CaM-kinase II. Phosphorylation may inhibit the interaction with DMD.
  • 細胞内局在Cell membrane > sarcolemma. Cell junction. Cytoplasm > cytoskeleton. In skeletal muscle, it localizes at the cytoplasmic side of the sarcolemmal membrane and at neuromuscular junctions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 59 kDa dystrophin-associated protein A1 acidic component 1 antibody
    • Acidic alpha 1 syntrophin antibody
    • Alpha 1 syntrophin antibody
    • Alpha-1-syntrophin antibody
    • dJ1187J4.5 antibody
    • Dystrophin associated protein A1 59kDa acidic component antibody
    • LQT12 antibody
    • OTTHUMP00000030650 antibody
    • Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody
    • SNT1 antibody
    • Snta1 antibody
    • SNTA1_HUMAN antibody
    • Syntrophin 1 antibody
    • Syntrophin-1 antibody
    • TACIP1 antibody
    see all

Anti-Syntrophin alpha 1 antibody (ab11187) 使用論文

This product has been referenced in:
  • Anderova M  et al. Altered astrocytic swelling in the cortex of a-syntrophin-negative GFAP/EGFP mice. PLoS One 9:e113444 (2014). WB ; Mouse . Read more (PubMed: 25426721) »
  • Koo T  et al. Delivery of AAV2/9-Microdystrophin Genes Incorporating Helix 1 of the Coiled-Coil Motif in the C-Terminal Domain of Dystrophin Improves Muscle Pathology and Restores the Level of a1-Syntrophin and a-Dystrobrevin in Skeletal Muscles of mdx Mice. Hum Gene Ther : (2011). IHC-Fr ; Mouse . Read more (PubMed: 21453126) »

See all 4 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (rat spinal cord cytosolic and membranous extract)
Loading amount 40 µg
Specification rat spinal cord cytosolic and membranous extract
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jun 21 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (skeletal muscle)
Specification skeletal muscle
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2%
Username

Dr. bruno Constantin

Verified customer

投稿 Mar 02 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"