製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Syntenin
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to internal sequence amino acids 109-158 of Human Syntenin (KQGIREVILC KDQDGKIGLR LKSIDNGIFV QLVQANSPAS LVGLRFGDQV)

  • ポジティブ・コントロール
    • HepG2 cell lysate. Human kidney tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

ターゲット情報

  • 機能
    Seems to function as an adapter protein. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA). May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway.
  • 組織特異性
    Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.
  • 配列類似性
    Contains 2 PDZ (DHR) domains.
  • 翻訳後修飾
    Phosphorylated on tyrosine residues.
  • 細胞内局在
    Cell junction > focal adhesion. Cell junction > adherens junction. Cell membrane. Endoplasmic reticulum membrane. Nucleus. Melanosome. Cytoplasm > cytosol. Cytoplasm > cytoskeleton. Mainly membrane-associated. Localized to adherens junctions, focal adhesions and endoplasmic reticulum. Colocalized with actin stress fibers. Also found in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MDA-9 antibody
    • MDA9 antibody
    • Melanoma differentiation-associated protein 9 antibody
    • Pro-TGF-alpha cytoplasmic domain-interacting protein 18 antibody
    • Scaffold protein Pbp1 antibody
    • SDCB1_HUMAN antibody
    • SDCBP antibody
    • ST1 antibody
    • SYCL antibody
    • Syndecan binding protein (syntenin) antibody
    • Syndecan binding protein 1 antibody
    • Syndecan-binding protein 1 antibody
    • Syntenin 1 antibody
    • Syntenin-1 antibody
    • TACIP18 antibody
    see all

画像

  • All lanes : Anti-Syntenin antibody (ab62530) at 1.25 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Syntenin with ab62530 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

参考文献

ab62530 has not yet been referenced specifically in any publications.

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