Anti-Syntenin 2 抗体 (ab83537)

製品の概要

  • 製品名Anti-Syntenin 2 antibody
    Syntenin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Syntenin 2
  • アプリケーション適用あり: ELISA, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (VAPVTGYSLG VRRAEIKPGV REIHLCKDER GKTGLRLRKV DQGLFVQLVQ) of Human Syntenin 2 (NP_056500; Q9H190-3).

  • ポジティブ・コントロール
    • Hela cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-Syntenin 2 antibody 画像

  • Anti-Syntenin 2 antibody (ab83537) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 23 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
  • ab83537 staining Syntenin 2 in the MCF7, human breast epithelial from Human mammary glands by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde or 100% Methanol, permeabilized with Digitonin and blocked with 1% BSA for 10 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 21°C. An Alexa Fluor®488-conjugated Donkey anti-mouse IgG polyclonal (1/300) was used as the secondary antibody. DAPI was Diluted 1/1000 and samples mounted in gel.

    See Abreview

Anti-Syntenin 2 antibody (ab83537) 使用論文

ab83537 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Various cell types, see image!)
Loading amount 50 µg
Specification Various cell types, see image!
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Prof. Ritva Tikkanen

Verified customer

投稿 Aug 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MCF7, human breast epithelial)
Specification MCF7, human breast epithelial
Fixative Paraformaldehyde
Permeabilization Yes - digitonin
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Prof. Ritva Tikkanen

Verified customer

投稿 Aug 22 2012

ab57063 : DISCOUNT CODE: 100%ABR-000
ab83537: DISCOUNT CODE: 100%ABR-0000

Expiration date: 04 October 2012

I am very pleased to hear you would like to accept our offer and test ab57063 and ab83537in IF and/or IP.Each code will...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"