製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Syntenin 2
  • 特異性
    This antibody is expected to recognise both human isoforms (NP_056500; NP_536737).
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Syntenin 2 aa 280-292 (C terminal).
    Sequence:

    LLHHTMDHSIPDA

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab38745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 4 - 6 µg/ml.

ターゲット情報

画像

  • ab38745 staining Syntenin in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized; antigen retrieval was by heat mediation in Tris EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/50) for 30 minutes at 20°C. An undiluted HRP-conjugated rabbit anti-goat IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab38745 (4µg/ml) staining of paraffin embedded Human Liver shows staining of cytoplasm in hepatocytes. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

プロトコール

参考文献

ab38745 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9,0
Sample
Human Tissue sections (Colon)
Specification
Colon
Permeabilization
Yes - Wash Buffer from Dako with Tween
Fixative
Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 Mar 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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