Anti-Syntaxin-binding protein 4 抗体 [EP302Y] (ab40674)

製品の概要

  • 製品名Anti-Syntaxin-binding protein 4 antibody [EP302Y]
    Syntaxin-binding protein 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP302Y] to Syntaxin-binding protein 4
  • 特異性This antibody is specific for Synip. It is predicted to detect the isoform 2 based an sequence analysis.
  • アプリケーション適用あり: WB, Flow Cyt, IHC-Pmore details
    適用なし: ICC or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C terminus of the PDZ domain of Human Synip.

  • ポジティブ・コントロール
    • Human skeletal lysate and human lung carcinoma tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/20000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/250 - 1/500.
  • 追加情報Is unsuitable for ICC or IP.
  • ターゲット情報

    • 機能Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose.
    • 配列類似性Contains 1 PDZ (DHR) domain.
      Contains 1 WW domain.
    • 翻訳後修飾Phosphorylated on Ser-99 by PKB/AKT2 after insulin treatment. Phosphorylation on Ser-99 abolishes the interaction with STX4A (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ16496 antibody
      • MGC149829 antibody
      • MGC50337 antibody
      • OTTHUMP00000082667 antibody
      • OTTHUMP00000227400 antibody
      • STX4-interacting protein antibody
      • STXB4_HUMAN antibody
      • Stxbp4 antibody
      • Synip antibody
      • Syntaxin 4 interacting protein antibody
      • Syntaxin 4-interacting protein antibody
      • Syntaxin binding protein 4 antibody
      • Syntaxin-binding protein 4 antibody
      see all

    Anti-Syntaxin-binding protein 4 antibody [EP302Y] 画像

    • ab40674 at a 1:250 dilution staining Synip in human lung carcinoma.
    • Anti-Syntaxin-binding protein 4 antibody [EP302Y] (ab40674) at 1/5000 dilution + human skeletal lysate

      Predicted band size : 61 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    Anti-Syntaxin-binding protein 4 antibody [EP302Y] (ab40674) 使用論文

    This product has been referenced in:
    • Taniai E  et al. Crosstalk between PTEN/Akt2 and TGFbeta signaling involving EGF receptor down-regulation during the tumor promotion process from the early stage in a rat two-stage hepatocarcinogenesis model. Cancer Sci 100:813-20 (2009). IHC-P ; Rat . Read more (PubMed: 19309364) »
    • Okada S  et al. Synip phosphorylation is required for insulin-stimulated Glut4 translocation. Biochem Biophys Res Commun 356:102-6 (2007). Read more (PubMed: 17336927) »

    See all 2 Publications for this product

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    The immunogen sequence of anti TRAIL antibody used in this kit corresponds to 114-281 of human TRAIL. It is more likely that it will detect your partial N-terminus (aa114-281) protein.

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