製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to Syntaxin 4
  • アプリケーション
    適用あり: WB, Flow Cyt, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant protein fragment with GST tag, corresponding to amino acids 19 - 120 of Human Syntaxin 4 (NP_004595)

  • ポジティブ・コントロール
    • HeLa cell lysate
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab184545 as a replacement.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 33 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration. Detection limit for ab77037 is approximately 1ng/ml as a capture antibody.

ターゲット情報

  • 機能
    Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.
  • 組織特異性
    Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation.
  • 配列類似性
    Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • NY REN 31 antigen antibody
    • p35 2 antibody
    • PLACENTAL antibody
    • Renal carcinoma antigen NY REN 31 antibody
    • Renal carcinoma antigen NY-REN-31 antibody
    • Stx4 antibody
    • STX4_HUMAN antibody
    • STX4A antibody
    • Syntaxin 4 antibody
    • syntaxin 4A (placental) antibody
    • Syntaxin 4A antibody
    • Syntaxin-4 antibody
    see all

画像

  • Anti-Syntaxin 4 antibody (ab77037) at 1/500 dilution + HeLa cell lysate at 25 µg

    Secondary
    Goat anit-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 33 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • Anti-Syntaxin 4 antibody (ab77037) at 1/500 dilution + recombinant protein Syntaxin 4 at 0.1 µg

    Secondary
    Goat anit-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 33 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • ab77037 tested in recombinant GST tagged protein, with a detection limit of approximately 1 ng/ml
  • ICC/IF image of ab77037 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77037, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HL60 cells stained with ab77037 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77037, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed in 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

参考文献

This product has been referenced in:
  • Verboogen DRJ  et al. Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation. Elife 6:N/A (2017). WB ; Human . Read more (PubMed: 28524818) »
  • Kioumourtzoglou D  et al. Insulin stimulates syntaxin4 SNARE complex assembly via a novel regulatory mechanism. Mol Cell Biol 34:1271-9 (2014). Read more (PubMed: 24469400) »

See all 3 Publications for this product

レビューと Q&A


Unfortunately, I cannot provide any images to back this up as the supplier of this antibody doesn't approve this. However, I would like to assure you that this antibody has been tested in very successfully in Raw 264.7 (Mouse leukaemic monocyte ma...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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