製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SYNRG
  • アプリケーション
    適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rhesus monkey
  • 免疫原

    antigen sequence, corresponding to amino acids 46-158 of Human SYNRG.

  • ポジティブ・コントロール
    • Human cerebral cortex tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    May play a role in endocytosis and/or membrane trafficking at the trans-Golgi network (TGN). May act by linking the adapter protein complex AP-1 to other proteins.
  • 配列類似性
    Contains 1 EH domain.
  • ドメイン
    The DFXDF motifs mediate the interaction with gamma-appendage subunits AP1G1 and AP1G2.
  • 細胞内局在
    Cytoplasm. Golgi apparatus > trans-Golgi network membrane. Associates with membranes via the adapter protein complex AP-1. Colocalizes with AP1G1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AP1 subunit gamma-binding protein 1 antibody
    • AP1GBP1 antibody
    • Gamma-synergin antibody
    • Synergin gamma antibody
    • SYNG antibody
    • Synrg antibody
    • SYNRG_HUMAN antibody
    see all

画像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121761 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121761 at 1/75 dilution staining SYNRG in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.

参考文献

ab121761 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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