製品の概要

  • 製品名Anti-Syndecan 4 antibody
    Syndecan 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Syndecan 4
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Cow, Dog, Human, Pig, Baboon, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Syndecan 4.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both STO and NIH3T3 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).

ターゲット情報

  • 機能Cell surface proteoglycan that bears heparan sulfate.
  • 組織特異性Expressed in epithelial and fibroblastic cells.
  • 配列類似性Belongs to the syndecan proteoglycan family.
  • 翻訳後修飾Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
  • 細胞内局在Membrane. Secreted. Shedding of the ectodomain produces a soluble form.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Amphiglycan antibody
    • MGC22217 antibody
    • OTTHUMP00000031788 antibody
    • ryudocan amphiglycan antibody
    • Ryudocan antibody
    • Ryudocan core protein antibody
    • SDC 4 antibody
    • Sdc4 antibody
    • SDC4_HUMAN antibody
    • SYND 4 antibody
    • SYND4 antibody
    • syndecan 4 antibody
    • syndecan 4 (amphiglycan, ryudocan) antibody
    • syndecan proteoglycan 4 antibody
    • Syndecan-4 antibody
    • Syndecan4 antibody
    see all

Anti-Syndecan 4 antibody 画像

  • All lanes : Anti-Syndecan 4 antibody (ab104568) at 1 µg/ml

    Lane 1 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

Anti-Syndecan 4 antibody (ab104568) 使用論文

ab104568 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - whole (cardiac)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 40 µg
Specification cardiac
Blocking step Licor as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Feb 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"