Rabbit polyclonal to Syndecan 4
Rat, Cow, Dog, Human, Pig, Baboon, Macaque monkey, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Syndecan 4.
This antibody gave a positive signal in both STO and NIH3T3 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).
Cell surface proteoglycan that bears heparan sulfate.
Expressed in epithelial and fibroblastic cells.
Belongs to the syndecan proteoglycan family.
Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
Membrane. Secreted. Shedding of the ectodomain produces a soluble form.
Information by UniProt
Western blot - Anti-Syndecan 4 antibody (ab104568)
All lanes :
Anti-Syndecan 4 antibody (ab104568) at 1 µg/ml
Lane 1 :
STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 :
NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
18 kDa (
why is the actual band size different from the predicted?
The band observed at 18 kDa could potentially be a cleaved form of Syndecan 4 due to the presence of a 21 amino acid signal peptide.
has not yet been referenced specifically in any publications.
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