製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Syndecan 3
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
  • 免疫原

    Synthetic peptide surrounding amino acid 427 of human Syndecan 3

  • ポジティブ・コントロール
    • Rat kidney tissue lysate

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab63932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Cell surface proteoglycan that may bear heparan sulfate (By similarity). May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
  • 組織特異性
    Expressed in the nervous system, the adrenal gland, and the spleen.
  • 配列類似性
    Belongs to the syndecan proteoglycan family.
  • 翻訳後修飾
    O-glycosylated within the Thr/Ser-rich region which could interact with lectin domains on other molecules.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • N-syndecan antibody
    • Sdc3 antibody
    • SDC3_HUMAN antibody
    • SDCN antibody
    • SYND3 antibody
    • Syndecan neural type antibody
    • syndecan proteoglycan 3 antibody
    • Syndecan-3 antibody
    see all

画像

  • Anti-Syndecan 3 antibody (ab63932) at 1 µg/ml + Rat kidney tissue lysate at 30 µg

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
  • ICC/IF image of ab63932 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63932, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Tinholt M  et al. Syndecan-3 and TFPI colocalize on the surface of endothelial-, smooth muscle-, and cancer cells. PLoS One 10:e0117404 (2015). Read more (PubMed: 25617766) »
  • Coulson-Thomas VJ  et al. Loss of corneal epithelial heparan sulfate leads to corneal degeneration and impaired wound healing. Invest Ophthalmol Vis Sci 56:3004-14 (2015). Read more (PubMed: 26024086) »

See all 5 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E12 embryo, transversal sections)
Specification
E12 embryo, transversal sections
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X100 0.1%
Blocking step
donkey serum+goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Aug 03 2012

Thank you for your reply.
I am happy to confirm that we are happy to extent the testing discount to the testing of IHC-Fr (PFA-fixed). And I agree that this is a good plan.
Please remember the testing discount code will also valid in case the...

Read More

DISCOUNT CODE for ab63932: 100%0000000
DISCOUNT CODE for ab113893: 100%000000
Ablaufdatum: 30. September 2012
Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab63932 und ab113893 in "whole mount IHC" zu testen, annehmen...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録