Anti-Syndecan 3 抗体 (ab36653)

製品の概要

  • 製品名Anti-Syndecan 3 antibody
    Syndecan 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Syndecan 3
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide derived from within residues 250 - 350 of Human Syndecan 3.

    (Peptide available as ab36652.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal for Western Blot in Mouse Brain Tissue lysate. This antibody gave a positive signal for Immunohistochemistry (FFPE) in Mouse Brain Tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab36653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 46 kDa).

ターゲット情報

  • 機能Cell surface proteoglycan that may bear heparan sulfate (By similarity). May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
  • 組織特異性Expressed in the nervous system, the adrenal gland, and the spleen.
  • 配列類似性Belongs to the syndecan proteoglycan family.
  • 翻訳後修飾O-glycosylated within the Thr/Ser-rich region which could interact with lectin domains on other molecules.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • N-syndecan antibody
    • Sdc3 antibody
    • SDC3_HUMAN antibody
    • SDCN antibody
    • SYND3 antibody
    • Syndecan neural type antibody
    • syndecan proteoglycan 3 antibody
    • Syndecan-3 antibody
    see all

Anti-Syndecan 3 antibody 画像

  • Anti-Syndecan 3 antibody (ab36653) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • IHC image of ab36653 staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36653, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Syndecan 3 antibody (ab36653) 使用論文

ab36653 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

If you don't mind I will place your comment in the 'Additional notes' section of your Abreview as I think it would be helpful for other researchers.

Thank you forparticipating in our testing discount program!

Thank you for submitting your Abreview and for your honest feedback.

As you had rated your experience in the review as 'Poor', I just wanted to follow up with you to get some more details as to what you were unhappy about regarding use of ab3...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (brain)
Loading amount 20 µg
Specification brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

投稿 Apr 03 2012

Thank you for your reply.


I do apologize as there was a misunderstanding on my part regarding this antibody. I am sorry that the product was not working for your mouse samples. As you stated, the antibody is tested and guaranteed for mo...

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Thank you for your reply.


I am sorry to hear that ab36653 did not work well in your rat samples. I am glad to hear you have submitted the Abreview. We appreciate your feedback!


Regarding an alternative product, we do not...

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I am very pleased to hear you would like to accept our offer and testab36653 in rat. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the &...

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Thank you very much for your interest in ab36653.

To our knowledge, ab36653 has not been tested in rat. Therefore, I can offer a discount off a future purchase if you buy ab36653 now, test it inrat and submit feedback to us in the form of an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"