製品の概要

  • 製品名
    Anti-Syndecan-1 antibody [B-A38]
    Syndecan-1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B-A38] to Syndecan-1
  • 特異性
    This antibody recognises a heparan sulfate rich membrane glycoprotein
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Fr, Flow Cyt, IHC-P, IHC-FoFr, Functional Studiesmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    U266 cell line

  • ポジティブ・コントロール
    • Bone Marrow
  • 特記事項

    This antibody stains all plasma cells and plasma cell lines. No staining of other leucocytes is seen in peripheral blood or bone marrow, although some ALL cell lines express the antigen weakly. Some staining of epithelial and endothelial cells may be seen in immunohistology.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab34164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr 1/100 - 1/500.
Flow Cyt Use 10µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 25053416
Functional Studies Use at an assay dependent concentration.

This product contains sodium azide, we recommend removal by dialysis for functional assays. 

ターゲット情報

画像

  • ab34164 staining Syndecan in human lung tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). 5µm thick sections of tissue were heated at 60°C overnight, deparaffinized with xylene and rehydrated with an ethanol series.

    Sections were stained with antibodies specific to syndecan-1 (ab34164) and EC-SOD both at a 1/500 dilution. Cy3 and Alexa Fluor® 488-conjugated secondary antibodies were used.

    Green - EC-SOD, Red - syndecan, Blue - nuclear stain, Yellow - co-localization.

  • Flow cytometry analysis of U266 cell line using ab34164

参考文献

This product has been referenced in:
  • Suhovskih AV  et al. Prostate cancer cells specifically reorganize epithelial cell-fibroblast communication through proteoglycan and junction pathways. Cell Adh Migr 11:39-53 (2017). ICC/IF ; Human . Read more (PubMed: 27111714) »
  • Oikari LE  et al. Data defining markers of human neural stem cell lineage potential. Data Brief 7:206-15 (2016). Read more (PubMed: 26958640) »

See all 14 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
3% BSA and 10% normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Rhesus monkey Tissue sections (colon)
Specification
colon
Permeabilization
Yes - 0.1% Triton-X-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 0.5%
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.25% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. Janelle Bentz

Verified customer

投稿 Sep 20 2012

Thank you for contacting us.
The immunogen for this clone was a whole cell line (U266) rather than a particular peptide. Unfortunately, we currently do not provide the cell line as a separate product.
I am very sorry I couldn’t help you furth...

Read More


I am very pleased to hear you would like to accept our offer and test ab34164 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Both citrate (low pH) and tris (high pH)
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Mar 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 21 2012

Thank you for contacting us.

Here are my suggestions for the targets you mentioned:

SMC alpha actin - ab5694
Calponin - ab46794
Myosin heavy chain - ab683 (heavy chain II) or ab682 (heavy chain I)
SM22 - ab14106 Read More

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録