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Our Abpromise guarantee covers the use of ab60199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 34 kDa).|
Image from Taylor DR et al, PLoS Pathog. 2009 Nov;5(11):e1000666. Epub 2009 Nov 20, Fig 6.Samples were prepared in dissociation buffer and boiled for 5 minutes. Proteins were resolved by SDS-PAGE using either 7–17% polyacrylamide gradient gels or 14.5% polyacrylamide gels. For western blot analysis, resolved proteins were transferred to Immobilon P polyvinylidene difluoride membrane. The membrane was blocked by incubation for 1 hour with phosphate-buffered saline containing 0.1% (v/v) Tween-20 and 5% (w/v) dried milk powder. Antibody incubations were performed in phosphate-buffered saline-Tween containing 2% (v/v) bovine serum albumin. Horseradish peroxidase-conjugated streptavidin was used at 1/2000.