Anti-Syndecan-1 抗体 (ab60199)
Key features and details
- Rabbit polyclonal to Syndecan-1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Syndecan-1 antibody
Syndecan-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Syndecan-1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide (Human) surrounding amino acid 298
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ポジティブ・コントロール
- Mouse tissue lysate
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 3643R Syndecan-1 Antibody. 3643R-100 is the same size as the 100 µg size of ab60199.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab60199の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 34 kDa).
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特記事項 |
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WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 34 kDa). |
ターゲット情報
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機能
Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. -
配列類似性
Belongs to the syndecan proteoglycan family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 20969 Mouse
- SwissProt: P18828 Mouse
- Unigene: 2580 Mouse
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別名
- CD_antigen antibody
- CD138 antibody
- CD138 antigen antibody
see all
画像
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Anti-Syndecan-1 antibody (ab60199) at 4 µg/ml + Mouse tissue lysate
Predicted band size: 34 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
The observed band size is larger than predicted due to haparan sulfate and chondroitin sulfate modifications. -
Anti-Syndecan-1 antibody (ab60199) at 1/2000 dilution + lysate prepared from ScN2a cells.
Secondary
peroxidase-conjugated secondary
Developed using the ECL technique.
Predicted band size: 34 kDa
Samples were prepared in dissociation buffer and boiled for 5 minutes. Proteins were resolved by SDS-PAGE using either 7–17% polyacrylamide gradient gels or 14.5% polyacrylamide gels. For western blot analysis, resolved proteins were transferred to Immobilon P polyvinylidene difluoride membrane. The membrane was blocked by incubation for 1 hour with phosphate-buffered saline containing 0.1% (v/v) Tween-20 and 5% (w/v) dried milk powder. Antibody incubations were performed in phosphate-buffered saline-Tween containing 2% (v/v) bovine serum albumin. Horseradish peroxidase-conjugated streptavidin was used at 1/2000.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab60199 は 9 報の論文で使用されています。
- Cao G et al. circCEP128 Knockdown Suppresses Bladder Cancer Progression via Regulating microRNA-515-5p/SDC1 Axis. Cancer Manag Res 13:2885-2896 (2021). PubMed: 33833571
- Yu CG et al. Inhibition of Bruton Tyrosine Kinase Reduces Neuroimmune Cascade and Promotes Recovery after Spinal Cord Injury. Int J Mol Sci 23:N/A (2021). PubMed: 35008785
- Jing Z et al. Phosphorylation of Dab2 is involved in inhibited VEGF-VEGFR-2 signaling induced by downregulation of syndecan-1 in glomerular endothelial cell. Cell Biol Int 44:894-904 (2020). PubMed: 31868265
- Li H et al. Interferon-induced mechanosensing defects impede apoptotic cell clearance in lupus. J Clin Invest 125:2877-90 (2015). PubMed: 26098211
- Boeddeker SJ et al. Syndecan-1 knockdown in endometrial epithelial cells alters their apoptotic protein profile and enhances the inducibility of apoptosis. Mol Hum Reprod N/A:N/A (2014). Human . PubMed: 24478463
- Chaterji S et al. Syndecan-1 regulates vascular smooth muscle cell phenotype. PLoS One 9:e89824 (2014). Mouse . PubMed: 24587062
- Park PJ et al. An investigative peptide-acyclovir combination to control herpes simplex virus type 1 ocular infection. Invest Ophthalmol Vis Sci 54:6373-81 (2013). PubMed: 23989188
- Cassano M et al. Alpha sarcoglycan is required for FGF-dependent myogenic progenitor cell proliferation in vitro and in vivo. Development 138:4523-33 (2011). WB ; Mouse . PubMed: 21903674
- Taylor DR et al. Glypican-1 mediates both prion protein lipid raft association and disease isoform formation. PLoS Pathog 5:e1000666 (2009). WB ; Mouse . PubMed: 19936054