Anti-Synaptotagmin VII 抗体 (ab121383)
Key features and details
- Rabbit polyclonal to Synaptotagmin VII
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Synaptotagmin VII antibody
Synaptotagmin VII 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Synaptotagmin VII -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
antigen, corresponding to amino acids 51-181 of Human Synaptotagmin VII.
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ポジティブ・コントロール
- Synaptotagmin VII-transfected HEK293T lysate; Human kidney tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121383の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. -
組織特異性
Expressed in a variety of adult and fetal tissues. -
配列類似性
Belongs to the synaptotagmin family.
Contains 2 C2 domains. -
ドメイン
The second C2 domain/C2B is responsible for SYNCRIP binding. -
細胞内局在
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9066 Human
- Omim: 604146 Human
- SwissProt: O43581 Human
- Unigene: 502730 Human
- Unigene: 684589 Human
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別名
- IPCA 7 antibody
- IPCA-7 antibody
- IPCA7 antibody
see all
画像
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All lanes : Anti-Synaptotagmin VII antibody (ab121383) at 1/250 dilution
Lane 1 : vector only transfected HEK293T lysate
Lane 2 : Synaptotagmin VII-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
10-20% SDS PAGE -
ab121383 at 1/25 dilution staining Synaptotagmin VII in Paraffin-embedded Human kidney tissue by Immunohistochemistry, showing strong cytoplasmic positivity, with a granular pattern, in cells in tubules.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab121383 は 2 報の論文で使用されています。
- Han Q et al. High SYT7 expression is associated with poor prognosis in human non-small cell lung carcinoma. Pathol Res Pract 216:153101 (2020). PubMed: 32825966
- Liu X et al. Synaptotagmin 7 in twist-related protein 1-mediated epithelial - Mesenchymal transition of non-small cell lung cancer. EBioMedicine 46:42-53 (2019). PubMed: 31395502