Anti-Synaptotagmin 1 抗体 (ab53484)

製品の概要

  • 製品名Anti-Synaptotagmin 1 antibody
    Synaptotagmin 1 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to Synaptotagmin 1
  • 特異性ab53484 recognizes Synaptotagmin 1.
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human, Apteronotus leptorhynchus
  • 免疫原

    Four synthetic peptides conjugated to KLH, corresponding to different regions of the Synaptotagmin 1 gene product, but are shared between the human (NP_005630, NCBI) and mouse (NP_033332, NCBI) sequences.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
  • 追加情報ICC/IF: 1/100.
    WB: 1/1000. Predicted molecular weight: 48 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
    • 配列類似性Belongs to the synaptotagmin family.
      Contains 2 C2 domains.
    • ドメインThe first C2 domain mediates Ca(2+)-dependent phospholipid binding.
      The second C2 domain mediates interaction with SV2A and STN2.
    • 細胞内局在Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Cytoplasm. Synaptic vesicles and chromaffin granules.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DKFZp781D2042 antibody
      • FLJ42519 antibody
      • p65 antibody
      • SVP65 antibody
      • Synaptotagmin 2 antibody
      • Synaptotagmin I antibody
      • Synaptotagmin II antibody
      • Synaptotagmin-1 antibody
      • SYT antibody
      • Syt1 antibody
      • SYT1_HUMAN antibody
      • Syt2 antibody
      • SytI antibody
      see all

    Anti-Synaptotagmin 1 antibody 画像

    Anti-Synaptotagmin 1 antibody (ab53484) 使用論文

    ab53484 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Apteronotus leptorhynchus Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization Yes - 0.3% Triton X-100
    Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
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    Verified customer

    投稿 Nov 26 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount 50 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing (4-15%)
    Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    Verified customer

    投稿 Nov 26 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"