This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Forebrain; Mouse Hippocampus; Mouse Cerebellum; Mouse Olfactory bulb; Rat Brain; Rat Hippocampus.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
Belongs to the synaptotagmin family. Contains 2 C2 domains.
The first C2 domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with SV2A and STN2.
The predicted molecular weight of Synaptotagmin 1 is 47 kDa (SwissProt), however we expect to observe a banding pattern at 60 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.