The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Minimum dilution 1/3000. Predicted molecular weight: 100 kDa.
Staining of synaptopodin in immunoblotting is specifically inhibited with the
synaptopodin immunizing peptide (rat, amino acids 184-202).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.
Expressed in cerebral cortex.
Belongs to the synaptopodin family.
O-glycosylated. Isoform 2 is phosphorylated on Ser-826, Thr-836, Ser-840 and Ser-871.
Cytoplasm > cytoskeleton. Cell junction > tight junction. Perikaryon. Cell projection > dendritic spine. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses.