製品の概要

  • 製品名Anti-Synaptophysin antibody [YE269]
    Synaptophysin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [YE269] to Synaptophysin
  • アプリケーション適用あり: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Donkey, Human
    交差が予測される動物種: Cow
  • 免疫原

    A synthetic peptide corresponding to residues in C terminus (cytoplasmic domain) of human Synaptophysin.

  • ポジティブ・コントロール
    • WB: PC12 cell lysate, human fetal brain, mouse brain, rat brain IF: PC12 cells. IHC: Human pancreas, mouse cerebral cortex, rat cerebral cortex, medullablastoma, lung neuroendocrine tumor
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:
    Anti-Synaptophysin antibody (Alexa Fluor® 488) [YE269] (ab196379)
    Anti-Synaptophysin antibody (Alexa Fluor® 647) [YE269] (ab196166)

    Anti-Synaptophysin antibody (HRP) [YE269] (ab195520)

    Anti-Synaptophysin antibody (Alexa Fluor® 594) [YE269] (ab206868)

    Anti-Synaptophysin antibody (Alexa Fluor® 555) [YE269] (ab206870)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/20000 - 1/100000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).Can be blocked with Synaptophysin peptide (ab189853).
ICC/IF 1/50.

For unpurified, use at 1/250.

IHC-Fr Use at an assay dependent concentration.
IHC-P 1/400 - 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ターゲット情報

  • 機能Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • 組織特異性Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • 関連疾患Mental retardation, X-linked, SYP-related
  • 配列類似性Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • ドメインThe calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • 翻訳後修飾Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • 細胞内局在Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Anti-Synaptophysin antibody [YE269] 画像

  • All lanes : Anti-Synaptophysin antibody [YE269] (ab32127) at 1/100000 dilution (purified)

    Lane 1 : Mouse brain
    Lane 2 : Rat brain

    Secondary
    HRP goat anti-rabbit at 1/1000 dilution

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescent staining of PC-12 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab32127 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counter stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor® 594 goat anti-mouse was used at a dilution of 1/500.

  • Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab32127 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab32127 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemical staining of paraffin embedded human pancreas with purified ab32127 at a dilution of 1/400. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Anti-Synaptophysin antibody [YE269] (ab32127) at 1/100000 dilution (purified) + Human fetal brain at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-Synaptophysin antibody [YE269] (ab32127) at 1/10000 dilution (unpurified) + PC12 cell lysate

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 21 kDa,22 kDa. We are unsure as to the identity of these extra bands.
  • Unpurified ab32127 showing positive staining in Medulloblastoma tissue.

  • Unpurified ab32127 showing positive staining in Lung neuroendocrine tumor tissue.

Anti-Synaptophysin antibody [YE269] (ab32127) 使用論文

This product has been referenced in:
  • Atzpodien EA  et al. Advanced Clinical Imaging and Tissue-based Biomarkers of the Eye for Toxicology Studies in Minipigs. Toxicol Pathol 44:398-413 (2016). Read more (PubMed: 26680760) »
  • Shin CH  et al. The BRAF kinase domain promotes the development of gliomas in vivo. Genes Cancer 6:9-18 (2015). IHC-P ; Mouse . Read more (PubMed: 25821557) »

See all 16 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH9
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% Normal buffered formalin
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投稿 Dec 05 2016

Application IHC - Wholemount
Sample Mouse Tissue (Diaphragm)
Specification Diaphragm
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投稿 Oct 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (neurons from iPS cells)
Gel Running Conditions Non-reduced Denaturing (12%)
Loading amount 20 µg
Specification neurons from iPS cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Jan 15 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Rat Tissue sections (Spinal cord)
Specification Spinal cord
Permeabilization Yes - 0.1% triton x-100
Fixative Paraformaldehyde
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投稿 Oct 06 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Donkey Tissue sections (brain slice)
Specification brain slice
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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投稿 Sep 08 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Cat Tissue sections (brainstem slice)
Specification brainstem slice
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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投稿 Sep 08 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample Chicken Tissue sections (Brain stem)
Specification Brain stem
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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投稿 Sep 08 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Sample Mouse Tissue sections (brainstem slice)
Specification brainstem slice
Permeabilization Yes - 0.5% triton
Fixative Paraformaldehyde
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投稿 Aug 18 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Sample Rat Tissue sections (brainstem slice)
Specification brainstem slice
Permeabilization Yes - 0.5% triton
Fixative Paraformaldehyde
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投稿 Aug 18 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Sample Human Cell (Human Cell (human iPS cell derived neurons))
Specification Human Cell (human iPS cell derived neurons)
Permeabilization Yes - see blocking buffer
Fixative Paraformaldehyde
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投稿 Jul 10 2014

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