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Our Abpromise guarantee covers the use of ab8049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
ab8049 staining Synaptophysin in mouse hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 1 hour at 24°C. Samples were incubated with primary antibody (1/100 in BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
ab8049 staining MAFA in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouseIgG polyclonal (1/300) was used as the secondary antibody.
The pictures show immunohistochemical staining of normal mucosa and neuroendocrine tumour of the small intestine. The antibody (ab8049) stains positive in a 1:10 dilution in nerve cells of the colon as well as some cells in normal colon mucosa and some neuroendocrine tumors of the digestive tract. Staining protocol: Mouse monoclonal to Synaptophysin (ab8049), diluted 1:10 in PBS plus BSA, 60 min at room temperature,
secondary antibody: HRP solution with anti mouse polyclonal antibodies, 30min. DAB staining 10 min; Hematoxylin 30 sec.
These images were kindly supplied as part of the review submitted by Karin Birkenkamp-Demtroeder.