RabMAb

Anti-Synaptophysin 抗体 [EP1098Y] (ab52636)

Abreviews (4)Q&A (8)Specific References (12)
Customer reviews and FAQs

製品の概要

  • 製品名Anti-Synaptophysin antibody [EP1098Y]
    Synaptophysin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1098Y] to Synaptophysin
  • アプリケーション適用あり: IHC-P, WB, IP, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues on Synaptophysin (Human)

  • ポジティブ･コントロール
    • Rat brain membrane. Human medullablastoma. PC12 cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Synaptophysin antibody (HRP) [EP1098Y] (ab204260)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
IP 1/50 - 1/100.
Flow Cyt 1/20 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/250 - 1/500.

ターゲット情報

  • 機能Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • 組織特異性Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • 関連疾患Mental retardation, X-linked, SYP-related
  • 配列類似性Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • ドメインThe calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • 翻訳後修飾Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • 細胞内局在Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Anti-Synaptophysin antibody [EP1098Y] 画像

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [EP1098Y] (ab52636)
    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [EP1098Y] (ab52636)
    PC12 cells labelled with ab52636 at 1/250 - 1/500 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin [EP1098Y] antibody (ab52636)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin [EP1098Y] antibody (ab52636)
    Human medulloblastoma labelled with ab52636 at 1/250 - 1/500 dilution
  • Western blot - Anti-Synaptophysin antibody [EP1098Y] (ab52636)
    Western blot - Anti-Synaptophysin antibody [EP1098Y] (ab52636)
    Anti-Synaptophysin antibody [EP1098Y] (ab52636) at 1/200000 dilution + Rat brain membrane

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Flow Cytometry - Anti-Synaptophysin antibody [EP1098Y] (ab52636)
    Flow Cytometry - Anti-Synaptophysin antibody [EP1098Y] (ab52636)
    Overlay histogram showing PC12 cells stained with ab52636 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52636, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-Synaptophysin antibody [EP1098Y] (ab52636) 使用論文

This product has been referenced in:
  • Curcio M  et al. Brain ischemia downregulates the neuroprotective GDNF-Ret signaling by a calpain-dependent mechanism in cultured hippocampal neurons. Cell Death Dis 6:e1645 (2015). Read more (PubMed: 25675305) »
  • Aligny C  et al. Ketamine alters cortical integration of GABAergic interneurons and induces long-term sex-dependent impairments in transgenic Gad67-GFP mice. Cell Death Dis 5:e1311 (2014). WB ; Mouse . Read more (PubMed: 24991763) »

See all 12 Publications for this product

ab52636 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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ab52636 の abreview を投稿する ab52636 に関する問い合わせをする

12 Abreviews or Q&A

10
Votes: Highest First

Immunocytochemistry/ Immunofluorescence abreview for Anti-Synaptophysin antibody [EP1098Y]

 Excellent
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse primary neurons, 2wks in culture)
Permeabilization Yes - 0.5% TX100
Specification mouse primary neurons, 2wks in culture
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Herr Dr. Vladimir Milenkovic

Verified customer

投稿 Nov 04 2016

Western blot abreview for Anti-Synaptophysin antibody [EP1098Y]

 Excellent
Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (brain)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 25 µg
Specification brain
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Herr Dr. Vladimir Milenkovic

Verified customer

投稿 Nov 04 2016

I would like to know which region of the Synaptophysin protein this antibody would bind to - intracellular or extracellular?


The immunogen is located in a cytoplasmic region of the protein.

We have bought your product, ab52636 and ab32127 (anti-synaptophysin) for immunofluorescence staining.

Before we stain our cells, we would like to stain PC12 cells as a positive control since I saw nice IF picture on the datasheet of both of...

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I believe undifferentiated PC12 cells should give you good results as a positive control for ab52636 and ab32127. The cycle of the PC12 cells in these images is unknown however, the cells are grown to 90-95% confluent before they are harvested.

IHC-P with human pancreas
sees neg. staining in islets, and pos. staining in acini - should be the opposite
AR: citrate buffer
Ab: 1/100, 1/1000 same pattern
block: H2O2, no protein block
2nd: OK
other Ab worked fine

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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ab52636 is showing weak/diffuse staining in IHC on primary rat cortical cultures.

Thank you for contacting us last week.

I am sorry that you have been experiencing problems with ab52636. Below is a copy of the protocol used to characterize the antibody in IHC. The antibody has not been specifically tested on primary mouse...

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Western blot abreview for Anti-Synaptophysin antibody [EP1098Y]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (ht1080)
Loading amount 100000 cells
Specification ht1080
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Abcam user community

Verified customer

投稿 May 23 2012

send information about testing discount program

Thank you for contacting us.

Here please find the information about our testing discount program. Below I also include information about our Abpromise guarantee.

For UNTESTED species and/or applications, we have established a testi...

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What is the concentration of lot GR27991-1 of ab52636?

Thank you for contacting Abcam. My colleague is out of the office but we have recieved the information that you requested so I thought I would pass that on to you. The concentration of lot GR27991-1 of ab52636 Anti-Synaptophysin antibody is 0.76mg/mL. ...

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The customer sent me two references to indicate that the molecular weight is 38kDa in the literature.why there is a difference? Looking forward to your reply.

Thank you for getting back to me. As I have mentioned in the previous email, there are many reasons to the differences but the most common factor is because of the gel percentage, intensity of current, etc which will cause the protein to run a bit ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"