製品の概要

  • 製品名
    Anti-Synaptophysin antibody
    Synaptophysin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Synaptophysin
  • 特異性
    This antibody reacts with a hydrophilic peptide sequence of synaptophysin.
  • アプリケーション
    適用あり: IHC-FoFr, IHC-Fr, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide (Human).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • 組織特異性
    Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • 関連疾患
    Mental retardation, X-linked, SYP-related
  • 配列類似性
    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • ドメイン
    The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • 翻訳後修飾
    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • 細胞内局在
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

画像

  • ICC/IF image of ab7837 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7837, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Sooy K  et al. Cognitive and Disease-Modifying Effects of 11ß-Hydroxysteroid Dehydrogenase Type 1 Inhibition in Male Tg2576 Mice, a Model of Alzheimer's Disease. Endocrinology 156:4592-603 (2015). WB ; Mouse . Read more (PubMed: 26305888) »
  • McClean PL & Hölscher C Liraglutide can reverse memory impairment, synaptic loss and reduce plaque load in aged APP/PS1 mice, a model of Alzheimer's disease. Neuropharmacology 76 Pt A:57-67 (2014). Read more (PubMed: 23973293) »

See all 5 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Vadivel Parthsarathy

Verified customer

投稿 Aug 10 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Vadivel Parthsarathy

Verified customer

投稿 Oct 15 2013

Thank you for contacting us.

Here please find the information about our testing discount program. Below I also include information about our Abpromise guarantee.

For UNTESTED species and/or applications, we have established a testi...

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IF. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in ret...

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This antibody has not yet been tested for cross reactivity with rat. However, it works well in IHC and is a polyclonal and will thus recognise a number of epitopes on the immunogen. So it is possible that it will cross react with rodent synaptophysin. ...

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The anitbody has not yet been tested for cross reativity with rat and mouse. However, it works well in IHC and is a polyclonal and will thus recognise a number of epitopes on the immunogen. I'd therefore predict that it will cross react with rodent syn...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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