Anti-Synaptogyrin 3 抗体 (ab106460)

Submit an Abreview Q&A (3)

製品の概要

  • 製品名
    Anti-Synaptogyrin 3 antibody
    Synaptogyrin 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Synaptogyrin 3
  • 由来種
    Rabbit
  • 特異性
    ab106460 does not cross-react with other Synaptogyrin family members
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A 19 amino acid peptide from near the C terminal of Human Synaptogyrin 3 (NP_004200).

  • ポジティブ･コントロール
    • WB: Mouse brain tissue lysate IHC-P: Rat brain tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (stable for up to 12 months).
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Involved in the positive regulation of dopamine transporter activity. Probably facilitates the physical and functional interactions of the transporter with the dopamine vesicular storage system, allowing a more efficient loading of the vesicles with extracellular dopamine after release.
  • 組織特異性
    Expressed in brain and placenta.
  • 配列類似性
    Belongs to the synaptogyrin family.
    Contains 1 MARVEL domain.
  • 細胞内局在
    Membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle. Cell junction > synapse. Found at the neuromuscular synapses.
  • Information by UniProt
  • 参照データベース
    • 別名
      • MGC:20003 antibody
      • MGC20003 antibody
      • OTTHUMP00000158899 antibody
      • SNG3_HUMAN antibody
      • Synaptogyrin 3 antibody
      • Synaptogyrin-3 antibody
      • Synaptogyrin3 antibody
      • SYNGR 3 antibody
      • SYNGR3 antibody
      see all

    画像

    • Western blot - Synaptogyrin 3 antibody (ab106460)
      Western blot - Synaptogyrin 3 antibody (ab106460)
      Lane 1 : Anti-Synaptogyrin 3 antibody (ab106460) at 1 µg/ml
      Lane 2 : Anti-Synaptogyrin 3 antibody (ab106460) at 2 µg/ml

      All lanes : Mouse brain tissue lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 25 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Synaptogyrin 3 antibody (ab106460)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Synaptogyrin 3 antibody (ab106460)
      ab106460, at 2.5 ug/ml, staining Synaptogyrin 3 in paraffin-embedded rat brain tissue.
    • Immunocytochemistry/ Immunofluorescence-Anti-Synaptogyrin 3 antibody(ab106460)
      Immunocytochemistry/ Immunofluorescence-Anti-Synaptogyrin 3 antibody(ab106460)
      Immunofluorescence of SYNGR3 in Rat Brain cells using ab106460 at 20 ug/ml.

    参考文献

    ab106460 has not yet been referenced specifically in any publications.

    ab106460 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    3 reviews or Q&A

    10
    Votes: Highest First

    I will attempt to answer your questions below:
    1. What's the species you were using to detect the proteins: human,
    mouse, etc. proteins with a tag? HEK-293 lysates (human clones with
    N-terminal V5-tag). Some abs from abcam (ab60332, ab80...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Please find the WB questionnaire below:
    1) Abcam product code ab: (ab15575, ab53532, ab96615 and ab106460)

    3) Description of the problem: the mentioned antibodies are
    non-specific i.e. several bands appear, none of which is the rig...

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    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for...

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    Product code: 15575
    Lot number: GR38115
    Inquiry: Dear Sir/Madam, I have previously made orders for primary antibodies (ref: #xxxxx)and #xxxx) and lately realised that they are non-specific. The tests were done by western blot on V5-tagged con...

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    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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