Anti-SYCP2L 抗体 (ab122224)
Key features and details
- Rabbit polyclonal to SYCP2L
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-SYCP2L antibody -
製品の詳細
Rabbit polyclonal to SYCP2L -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
antigen sequence: TSLICVIEDF FDTALIISRS SSEGKIQMLD SFLLSLGFLV TEKTVNHLLQ QEGLKTFNCI LHAVPREERK KFPLSEGMCH LMKDLARTLL TVGDYDQQV, corresponding to amino acids of Human SYCP2L.
-
ポジティブ・コントロール
- Human cerebral cortex tissue; A431 cells; RT 4, U 251 MG, Human liver and tonsil lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122224の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
1/250 - 1/500.
|
|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
特記事項 |
---|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/250 - 1/500. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
ターゲット情報
-
組織特異性
Expressed in the ovary (at protein level). -
配列類似性
Belongs to the SYCP2 family. -
細胞内局在
Nucleus. Detected in nuclear granules and sizable aggregates. - Information by UniProt
-
参照データベース
- Entrez Gene: 221711 Human
- SwissProt: Q5T4T6 Human
- Unigene: 729128 Human
-
別名
- 145 kDa nucleolar protein homolog antibody
- C6orf177 antibody
- dJ62D2.1 antibody
see all
画像
-
ab122224, at 1/200 dilution, staining SYCP2L in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
-
ab122224, at 4 µg/ml, staining SYCP2L in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
-
All lanes : Anti-SYCP2L antibody (ab122224) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
ab122224 appears to be recognizing isoform 2 of Human SYCP2L (34kDa).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab122224 は論文での使用が確認できていません。