製品の概要

  • 製品名Anti-SYBL1 antibody [158.2]
    SYBL1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [158.2] to SYBL1
  • アプリケーション適用あり: Flow Cyt, ICC/IF, WB, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Dog
  • 免疫原

    Recombinant full SYBL1 protein (Human)

  • ポジティブ・コントロール
    • extract from rat brain

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab36195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 19138172
WB 1/500 - 1/2000. Predicted molecular weight: 24 kDa.
IHC-Fr 1/10 - 1/400.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.
  • 組織特異性Detected in all tissues tested.
  • 配列類似性Belongs to the synaptobrevin family.
    Contains 1 longin domain.
    Contains 1 v-SNARE coiled-coil homology domain.
  • 細胞内局在Cytoplasmic vesicle > secretory vesicle membrane. Golgi apparatus > trans-Golgi network membrane. Late endosome membrane. Lysosome membrane. Endoplasmic reticulum membrane. Cytoplasmic vesicle > phagosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ53045 antibody
    • FLJ53762 antibody
    • FLJ54296 antibody
    • HGNC:11486 antibody
    • OTTHUMP00000024258 antibody
    • OTTHUMP00000024259 antibody
    • OTTHUMP00000225953 antibody
    • SYBL 1 antibody
    • SYBL1 antibody
    • Synaptobrevin like 1 antibody
    • Synaptobrevin-like protein 1 antibody
    • Tetanus insensitive VAMP antibody
    • Tetanus neurotoxin insensitive VAMP antibody
    • Tetanus-insensitive VAMP antibody
    • TI VAMP antibody
    • Ti-VAMP antibody
    • TIVAMP antibody
    • VAMP-7 antibody
    • VAMP7 antibody
    • VAMP7_HUMAN antibody
    • Vesicle-associated membrane protein 7 antibody
    see all

Anti-SYBL1 antibody [158.2] 画像

  • Anti-SYBL1 antibody [158.2] (ab36195) at 1/500 dilution + Tissue lysates prepared from rat brain

    Predicted band size : 24 kDa
  • Overlay histogram showing PC12 cells stained with ab36195 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36195, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

Anti-SYBL1 antibody [158.2] (ab36195) 使用論文

This product has been referenced in:
  • Pirooz SD  et al. UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs. Proc Natl Acad Sci U S A 111:2716-21 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24550300) »
  • Kukic I  et al. Zn2+ efflux through lysosomal exocytosis prevents Zn2+-induced toxicity. J Cell Sci 127:3094-103 (2014). Read more (PubMed: 24829149) »

See all 12 Publications for this product

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Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Chinese Hamster Cell (Ovary (CHO-K1 - tissue culture))
Specification Ovary (CHO-K1 - tissue culture)
Fixative Formaldehyde
Permeabilization Yes - TX-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
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Mrs. Diana Thomas

Verified customer

投稿 Jun 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"