SWAP70 overexpression 293T lysate (whole cell) (ab94234)

製品の概要

  • 製品名SWAP70 overexpression 293T lysate (whole cell)
  • 製品の詳細
    SWAP70 overexpression lysate
  • 特記事項ab94234 is a 293T cell transfected lysate in which Human SWAP70 has been transiently over-expressed using a pCMV-SWAP70 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 背景Function: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. Tissue specificity: Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760). Similarity: Contains 1 PH domain. Domain: The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding. PTM: Tyrosine-phosphorylated.

アプリケーション

Our Abpromise guarantee covers the use of ab94234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

SWAP70 overexpression 293T lysate (whole cell) 画像

  • ab94234 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-SWAP70 antibody (ab89532) at 1/500 dilution

    Lane 1 : SWAP70 overexpression 293T lysate (whole cell) (ab94234)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

SWAP70 overexpression 293T lysate (whole cell) (ab94234) 使用論文

ab94234 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"