製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to SWAP70
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide: EEREKNWKEKKTTE, corresponding to C terminal amino acids 572-585 of Human SWAP70.

  • ポジティブ・コントロール
    • Daudi lysate.
  • 特記事項
    Principal Names – SWAP70; SWAP-70 protein; SWAP-70; KIAA0640; KIAA0640 protein. Official Gene Symbol - SWAP70 (interim symbol). GenBank Accession Number – XP_049197. LocusLink ID - 23075

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    Western Blot – Approx 70kDa band seen in Daudi lysate. Recommended for use at 1.0-3.0ug/ml.
  • ターゲット情報

    • 機能
      Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
    • 組織特異性
      Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760).
    • 配列類似性
      Contains 1 PH domain.
    • ドメイン
      The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
    • 翻訳後修飾
      Tyrosine-phosphorylated.
    • 細胞内局在
      Cytoplasm. Cell membrane. Nucleus. Cell projection > lamellipodium. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively extending lamellipodia.
    • Information by UniProt
    • 参照データベース
    • 別名
      • KIAA0640 antibody
      • 70kDa antibody
      • AV235546 antibody
      • FLJ39540 antibody
      • HSPC321 antibody
      • SWA 70 protein antibody
      • SWAP 70 antibody
      • SWAP switching B-cell complex 70kDa subunit antibody
      • SWAP-70 antibody
      • SWAP70 antibody
      • Switch associated protein 70 antibody
      • Switch-associated protein 70 antibody
      • SWP70_HUMAN antibody
      see all

    Anti-SWAP70 antibody 画像

    • ab2511 staining (2µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.

    プロトコール

    Anti-SWAP70 antibody (ab2511) 使用論文

    ab2511 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab2511.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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