SW1353 whole cell lysate (ab7915)

製品の概要

  • 製品名SW1353 whole cell lysate
  • 特記事項Cell line: SW1353 (Human humerus chondrosarcoma, fibroblast-like).
    Growth media: DMEM and 10% FBS (Fetal bovine serum).


    SW1353 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 6.80
    Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • 精製度Whole Cell Lysate
  • ライゼート 備考SW1353 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究分野
  • 背景The SW1353 chondrosarcoma cell line is considered to be a model for studies of the transcription factor Sox9, an important determinant of chondrocyte differentiation, including the regulation of type II collagen (Col2) and aggrecan gene expression. Cells are used to study mechanisms of joint inflammation and cartilage destruction.

アプリケーション

Our Abpromise guarantee covers the use of ab7915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. SW1353 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

SW1353 whole cell lysate (ab7915) 使用論文

ab7915 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab7915.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"