製品の概要

  • 製品名Anti-SV40 VP2 + VP3 antibody
  • 製品の詳細
    Rabbit polyclonal to SV40 VP2 + VP3
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Simian Virus 40 (SV40)
  • 免疫原

    Recombinant fusion protein: MAVDLYRPDD YYDILFPGVQ TFVHSVQYLD PRHWGPTLFN AISQAFWRVI QNDIPRLTSQ ELERRTQRYL RDSLARFLEE TTWTVINAPV NWYNSLQDYY STLSPIRPTM VRQVANREGL QISFGHTYDN IDEADSIQQV TERWEAQSQS PNVQSGEFIE KFEAPGGANQ RTAPQWMLPL LLGLYGSVTS ALKAYEDGPN KKKRKLSRGS SQKTKGTSAS AKARHKRRNR SSRS

  • 特記事項


    This antibody recognises two of the SV40 capsid proteins - VP2 and VP3

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 38,26 kDa.
ICC/IF Use at an assay dependent dilution. PubMed: 19104450

ターゲット情報

  • 関連性There are three simian virus 40 (SV40) capsid proteins - VP1, VP2 and VP3. VP1 assembles into pentamers which have a central cavity that contains a copy of one of the other capsid proteins, VP2 or VP3. This complex is then imported into the cell nucleus. Here, 72 of the complexes assemble around the newly synthesised viral genome to form the icosahedral capsid.
  • 参照データベース

    Anti-SV40 VP2 + VP3 antibody 画像

    • All lanes : Anti-SV40 VP2 + VP3 antibody (ab53983) at 1/5000 dilution

      Lane 1 : BK virus infected 293T cell lysate
      Lane 2 : Mock 293T cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 38,26 kDa
      Observed band size : 27,38 kDa (why is the actual band size different from the predicted?)


      Exposure time : 6 minutes

      This image is courtesy of an anonymous Abreview

      See Abreview



    • Predicted band size : 38,26 kDa
      SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3

    Anti-SV40 VP2 + VP3 antibody (ab53983) 使用論文

    This product has been referenced in:
    • Zins SR  et al. The human alpha defensin HD5 neutralizes JC polyomavirus infection by reducing endoplasmic reticulum traffic and stabilizing the viral capsid. J Virol 88:948-60 (2014). Read more (PubMed: 24198413) »
    • Henriksen S  et al. The human fetal glial cell line SVG p12 contains infectious BK polyomavirus. J Virol 88:7556-68 (2014). Simian Virus 40 (SV40) . Read more (PubMed: 24760884) »

    See all 4 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 15 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (BK virus infected 293TT cells)
    Specification BK virus infected 293TT cells
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 18 2014

    Application Western blot
    Loading amount 150000 cells
    Gel Running Conditions Reduced Denaturing (4-15%)
    Sample Simian Virus 40 (SV40) Cell lysate - nuclear (Sf9 insect cells)
    Specification Sf9 insect cells
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 05 2013

    I am so happy to hear that the replacement worked!  I wish you continued luck with your reseach and please do not hesitate to contact me if you should need any further assistance.  Have a great weekend!

    Thank you for your reply. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please contact ou...

    Read More

    Thank you for contacting Abcam regarding ab53983. The correct immunogen sequence is: MAVDLYRPDDYYDILFPGVQTFVHSVQYLDPRHWGPTLFNAISQAFWRVIQNDIPRLTSQELERRTQRYLRDSLARFLEETTWTVINAPVNWYNSLQDYYSTLSPIRPTMVRQVANREGLQISFGHTYDNIDEADSIQQVTERWEAQSQSPNVQSGEFIEKFEAPGG...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"