Anti-SV40 VP1 抗体 (ab53977)
Key features and details
- Rabbit polyclonal to SV40 VP1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Simian Virus 40
- Isotype: IgG
製品の概要
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製品名
Anti-SV40 VP1 antibody -
製品の詳細
Rabbit polyclonal to SV40 VP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IFmore details -
種交差性
交差種: Simian Virus 40 -
免疫原
Recombinant fusion protein
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53977の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/10000. Predicted molecular weight: 40 kDa.
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IP |
Use at an assay dependent dilution.
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ICC/IF |
1/1000 - 1/5000.
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特記事項 |
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WB
1/10000. Predicted molecular weight: 40 kDa. |
IP
Use at an assay dependent dilution. |
ICC/IF
1/1000 - 1/5000. |
ターゲット情報
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関連性
VP1 is one of three simian virus 40 (SV40) capsid proteins. VP1 assembles into pentamers which have a central cavity that contains a copy of one of the other capsid proteins, VP2 or VP3. This complex is then imported into the cell nucleus. Here, 72 of the complexes assemble around the newly synthesised viral genome to form the icosahedral capsid. -
参照データベース
- SwissProt: P03087 Simian Virus 40
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別名
- Capsid protein VP1 antibody
画像
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All lanes : Anti-SV40 VP1 antibody (ab53977) at 1/5000 dilution
Lane 1 : BK virus infected 293TT whole cell lysate
Lane 2 : Mock infected 293TT whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
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SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3
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ab53977 detecting VP1 by Immunoprecipitation. Mock or SV40-transfected CV1 cells were lysed with RIPA buffer. 100 µg total protein from each lysate was pre-cleared for 1 hour and then immunoprecipitated with alpha-VP1 1/1000 overnight at 4°C. Input and IP samples were run on an SDS gel and detected with alpha-VP1 diluted 1/10,000 using the ECL method.
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ab53977 at 1/1000 dilution, staining SV40 VP1 in transfected cells. Cells were transfected with SV40 or mock-transfected, fixed for 5 minutes after adsorption in 100% methanol, blocked and stained with the antibody for 1 hour at room temperature. Cells were washed and stained with an Alexa Fluor® 488-conjugated anti-rabbit antibody. DAPI was used to counterstain the nuclei.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (16)
ab53977 は 16 報の論文で使用されています。
- Spriggs CC et al. Components of the LINC and NPC complexes coordinately target and translocate a virus into the nucleus to promote infection. PLoS Pathog 18:e1010824 (2022). PubMed: 36067270
- Sikorski M et al. Non-permissive human conventional CD1c+ dendritic cells enable trans-infection of human primary renal tubular epithelial cells and protect BK polyomavirus from neutralization. PLoS Pathog 17:e1009042 (2021). PubMed: 33592065
- Bagchi P et al. Lunapark-dependent formation of a virus-induced ER exit site contains multi-tubular ER junctions that promote viral ER-to-cytosol escape. Cell Rep 37:110077 (2021). PubMed: 34879280
- Motamedi N et al. SV40 Polyomavirus Activates the Ras-MAPK Signaling Pathway for Vacuolization, Cell Death, and Virus Release. Viruses 12:N/A (2020). PubMed: 33028008
- Tsai K et al. Addition of m6A to SV40 late mRNAs enhances viral structural gene expression and replication. PLoS Pathog 14:e1006919 (2018). PubMed: 29447282