製品の概要

  • 製品名
    Anti-SV40 VP1 antibody
  • 製品の詳細
    Rabbit polyclonal to SV40 VP1
  • アプリケーション
    適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Simian Virus 40
  • 免疫原

    Recombinant fusion protein

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Predicted molecular weight: 40 kDa.
IP Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.

ターゲット情報

  • 関連性
    VP1 is one of three simian virus 40 (SV40) capsid proteins. VP1 assembles into pentamers which have a central cavity that contains a copy of one of the other capsid proteins, VP2 or VP3. This complex is then imported into the cell nucleus. Here, 72 of the complexes assemble around the newly synthesised viral genome to form the icosahedral capsid.
  • 別名
    • Capsid protein VP1 antibody

画像

  • All lanes : Anti-SV40 VP1 antibody (ab53977) at 1/5000 dilution

    Lane 1 : BK virus infected 293TT whole cell lysate
    Lane 2 : Mock infected 293TT whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    See Abreview



  • Predicted band size : 40 kDa
    SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3
  • ab53977 detecting VP1 by Immunoprecipitation. Mock or SV40-transfected CV1 cells were lysed with RIPA buffer. 100 µg total protein from each lysate was pre-cleared for 1 hour and then immunoprecipitated with alpha-VP1 1/1000 overnight at 4°C. Input and IP samples were run on an SDS gel and detected with alpha-VP1 diluted 1/10,000 using the ECL method.
  • ab53977 at 1/1000 dilution, staining SV40 VP1 in transfected cells. Cells were transfected with SV40 or mock-transfected, fixed for 5 minutes after adsorption in 100% methanol, blocked and stained with the antibody for 1 hour at room temperature. Cells were washed and stained with an Alexa Fluor® 488-conjugated anti-rabbit antibody. DAPI was used to counterstain the nuclei.

参考文献

This product has been referenced in:
  • Raja R  et al. HIV-1 Tat potently stabilises Mdm2 and enhances viral replication. Biochem J 474:2449-2464 (2017). WB ; Human . Read more (PubMed: 28468838) »
  • Wharton KA  et al. JC Polyomavirus Abundance and Distribution in Progressive Multifocal Leukoencephalopathy (PML) Brain Tissue Implicates Myelin Sheath in Intracerebral Dissemination of Infection. PLoS One 11:e0155897 (2016). Read more (PubMed: 27191595) »

See all 10 Publications for this product

レビューと Q&A

Abreviews
Application
ELISA
Sample
Simian Virus 40 Serum (Vector particles produced from packaging cells. Vi)
Specification
Vector particles produced from packaging cells. Vi
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 25°C
Type
Direct
Username

Miguel Garcia

Verified customer

投稿 Jan 24 2017

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (BK virus infected 293TT cells)
Specification
BK virus infected 293TT cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Nov 18 2014

Thank you for your enquiry and for your patience. We aim to get back to customers within 24 hours and I am sorry this did not happen on this occasion.

I have attached the MSDS for ab53977. This is now also available on the datasheet.
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Hello!

The supplier of the antibody was unable to provide a concentration, although I can tell you for rabbit antibodies typically the IgG is 12 - 15 mg/ml. If you did need a more specific concentration you could purify the antibody with a ki...

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Thank you for your enquiry. I can confirm that ab53977 SV40 VP1 antibody detects infecting SV40 in the cell during the first hours of infection, before new VP1 is expressed from the incoming virus. I am sorry it has not yet been tested tested i...

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Thank you for your enquiry. I have received confirmation from the originator of the product that this ab53977 do cross-react with JC and BK, and in-fact give very good signals with pathological specimens. However, it cannot be used to distinguish b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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