製品の概要

  • 製品名Anti-SV2A antibody
    SV2A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SV2A
  • アプリケーション適用あり: IHC-FoFr, IHC-P, WB, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat SV2A.

    (Peptide available as ab32998.)

  • ポジティブ・コントロール
    • Mouse or Rat Brain Tissue Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/1000.
IHC-P 1/200.
WB Use at an assay dependent concentration. Detects a band of approximately 82-92 kDa (predicted molecular weight: 83 kDa).
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles.
  • 配列類似性Belongs to the major facilitator superfamily.
  • 翻訳後修飾Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1.
    N-glycosylated.
  • 細胞内局在Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Enriched in chromaffin granules, not present in adrenal microsomes. Associated with both insulin granules and synaptic-like microvesicles in insulin-secreting cells of the pancreas.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA0736 antibody
    • OTTHUMP00000014065 antibody
    • SV2 antibody
    • Sv2a antibody
    • SV2A_HUMAN antibody
    • Synaptic vesicle glycoprotein 2 antibody
    • Synaptic vesicle glycoprotein 2A antibody
    • Synaptic vesicle protein 2a antibody
    see all

Anti-SV2A antibody 画像

  • Lane 1 : Marker
    Lanes 2 - 4 : Anti-SV2A antibody (ab32942) at 1/500 dilution

    Lane 1 : As above
    Lane 2 : Mouse Brain Tissue Lysate at 20 µg
    Lane 3 : Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg
    Lane 4 : Rat Brain Tissue Lysate at 20 µg

    Secondary
    Lanes 2 - 4 : IR Dye 680 Conjugated Goat anti-Rabbit IgG (H+L) at 1/15000 dilution


    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 82 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa. We are unsure as to the identity of these extra bands.
  • SV2A was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to SV2A and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32942.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 82kDa: SV2A
  • Immunostaining for SV2A in the rat hippocampus obtained with the antibody ab32942 (1:1000), using direct fluorescence. Please see accompanying abreview for additional information.

    See Abreview

Anti-SV2A antibody (ab32942) 使用論文

This product has been referenced in:
  • Chiang CK  et al. The proteomic landscape of the suprachiasmatic nucleus clock reveals large-scale coordination of key biological processes. PLoS Genet 10:e1004695 (2014). WB ; Mouse . Read more (PubMed: 25330117) »
  • Talos DM  et al. Antiepileptic effects of levetiracetam in a rodent neonatal seizure model. Pediatr Res 73:24-30 (2013). WB, ICC/IF ; Rat . Read more (PubMed: 23138400) »

See all 4 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A
Sample Human Cell lysate - whole cell (SH-5YSY differentiated into neurons)
Specification SH-5YSY differentiated into neurons
Total protein in input 30 µg
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Abcam user community

Verified customer

投稿 Jan 29 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (SH-5YSY)
Specification SH-5YSY
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

投稿 Jan 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain- Hippocampus)
Specification Brain- Hippocampus
Fixative Formaldehyde
Antigen retrieval step Other
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 22°C
Username

Ms. Gemma Lace

Verified customer

投稿 Aug 03 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

投稿 Oct 13 2008

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

投稿 Oct 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (cultured hippocampal neurons)
Specification cultured hippocampal neurons
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% Triton X-100 for 5 min
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Aug 16 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"