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Synthetic peptide within Human Survivin aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O15392
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab76424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Signal amplification system might be necessary or the use of polymerized HRP secondary antibodies.
For unpurified use at 1/250 - 1/500.
|WB||1/5000 - 1/20000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).|
For unpurified use at 1:10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
For unpurified use at 1/50.
|ICC/IF||1/100 - 1/250.|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to Survivin (ab27468).
For sandwich ELISA, use this antibody as detection at 0.5µg/ml with ab27468 as capture.
Immunofluorescent staining of MCF-7 cells labelling Bcl-2 with purified ab76424 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab7291, a mouse anti-tubulin (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary antibody. Nuclei counterstained with DAPI (blue).
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Unpurified ab76424 showing positive staining in Fetal kidney tissue.
Overlay histogram showing HeLa cells stained with unpurified ab76424 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76424, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.
Unpurified ab76424 at 1/250 dilution staining Survivin in human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Unpurified ab76424 at 1/250 dilution staining Survivin in human urinary bladder carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Unpurified ab76424 showing positive staining in breast carcinoma tissue.
Unpurified ab76424 showing positive staining in cervical carcinoma tissue.
Unpurified ab76424 showing positive staining in fetal liver tissue.
Unpurified ab76424 showing positive staining in normal tonsil tissue.
Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling Survivin with unpurified ab76424 at 1/100. Cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature. Cells were incubated with the primary antibody overnight. An Alexa Fluor® 555-conjugated anti-rabbit IgG was used as the secondary antibody. Left - Survivin, Right - DAPI.
ab76424 staining Survivin in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. Ab150081 (1/200) was used as the secondary antibody.