製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Survivin
  • アプリケーション
    適用あり: Sandwich ELISA, ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog
  • 免疫原

    Synthetic peptide:

    (C)VRRAIEQLAAMD

    , corresponding to C terminal amino acids 131-142 of Human Survivin

  • ポジティブ・コントロール
    • Human T cell line MOLT4 lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit monoclonal [EP2880Y] to Survivin (ab76424). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab76424 as Detection.
ELISA 1/16000.
WB Use a concentration of 0.01 - 0.5 µg/ml. Detects a band of approximately 18 kDa.

ターゲット情報

  • 機能
    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • 組織特異性
    Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • 配列類似性
    Belongs to the IAP family.
    Contains 1 BIR repeat.
  • 発生段階
    Expression is cell cycle-dependent and peaks at mitosis.
  • ドメイン
    The BIR repeat is necessary and sufficient for HBXIP binding.
  • 翻訳後修飾
    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • 細胞内局在
    Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • API4 antibody
    • Apoptosis inhibitor 4 antibody
    • Apoptosis inhibitor survivin antibody
    • Apoptosis inhibitor4 antibody
    • Baculoviral IAP repeat containing 5 antibody
    • Baculoviral IAP repeat containing protein 5 antibody
    • Baculoviral IAP repeat-containing protein 5 antibody
    • BIRC 5 antibody
    • BIRC5 antibody
    • BIRC5_HUMAN antibody
    • EPR 1 antibody
    • IAP4 antibody
    • Survivin variant 3 alpha antibody
    • SVV antibody
    • TIAP antibody
    see all

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参考文献

This product has been referenced in:
  • Zhang S  et al. Targeted inhibition of survivin with YM155 promotes apoptosis of hypoxic human pulmonary arterial smooth muscle cells via the upregulation of voltage-dependent K? channels. Mol Med Rep 13:3415-22 (2016). ICC . Read more (PubMed: 26957114) »

See 1 Publication for this product

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