製品の概要

  • 製品名Anti-Surfactant Protein A antibody [6F10]
    Surfactant Protein A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6F10] to Surfactant Protein A
  • 特異性ab51891 is specific for human pulmonary Surfactant Protein A
  • アプリケーション適用あり: ICC/IF, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Native Surfactant Protein A purified from bronchioaveolar lavage from proteinosis patients.

  • ポジティブ・コントロール
    • Human lung tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 21421565
WB 1/1000. Predicted molecular weight: 26 kDa.
ELISA 1/16000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 細胞内局在Secreted protein; extracellular space.
  • 参照データベース
  • 別名
    • 35 kDa pulmonary surfactant-associated protein antibody
    • Alveolar proteinosis protein antibody
    • COLEC4 antibody
    • Collectin 4 antibody
    • PSAP antibody
    • PSPA antibody
    • Pulmonary surfactant apoprotein antibody
    • Pulmonary surfactant-associated protein A1 antibody
    • SFTP1 antibody
    • SFTPA antibody
    • SFTPA1 antibody
    • SFTPA1B antibody
    • SP A antibody
    • SP A1 antibody
    see all

Anti-Surfactant Protein A antibody [6F10] 画像

  • ab51891 staining sections of human lung at 1:3000 dilution.  Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.
  • All lanes : Anti-Surfactant Protein A antibody [6F10] (ab51891) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa,63 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 150 seconds
  • ab51891 staining sections of human lung at 1:3000 dilution.  Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.

Anti-Surfactant Protein A antibody [6F10] (ab51891) 使用論文

This product has been referenced in:
  • Somarajan SR  et al. Annexin A2 mediates Mycoplasma pneumoniae community-acquired respiratory distress syndrome toxin binding to eukaryotic cells. MBio 5:N/A (2014). WB ; Human . Read more (PubMed: 25139904) »
  • Zhou J  et al. Biological features of novel avian influenza A (H7N9) virus. Nature 499:500-3 (2013). Read more (PubMed: 23823727) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Rabbit Tissue lysate - whole (lung)
Loading amount 25 µg
Specification lung
Gel Running Conditions Non-reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Mun-Suk Park

Verified customer

投稿 Nov 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"