The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 179 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
1/2000 - 1/5000.
Surface protein G (SasG) promotes adhesion of bacterial cells to human squamous nasal epithelial cells, a phenomenon which is likely to be important in nasal colonization. It forms short, extremely dense and thin fibrils all over the bacterial surface. SasG does not bind to either buccal cells or non-differentiated keratinocytes. SasG promotes cellular aggregation leading to biofilm formation. SasG is a secreted, cell wall protein. It is produced during infection of the human host. SasG does not bind to immobilized fibrinogen, fibronectin, IgG, human epidermal keratin, collagen, vWF, laminin, heparan sulfate and submaxillary mucin. When sasG is expressed at high levels, it inhibits adhesion of Staphylococcus aureus to the ligands fibrinogen, fibronectin, cytokeratin 10 and IgG. This inhibitory effect depends on the number of G5 repeats present in the protein, since shorter variants do not present this phenotype. Biofilm formation is ica-independent, relying only on the level of expression of the protein and the number of G5 repeats.