The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Belongs to the Mediator complex subunit 21 family.
Mediator of RNA polymerase II transcription subunit 21 antibody
RNA polymerase II holoenzyme component SRB7 antibody
RNAPII complex component SRB7 antibody
SRB7 suppressor of RNA polymerase B homolog antibody
Anti-Surb7 antibody 画像
ChIP - Anti-Surb7 antibody (ab123052)
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Western blot - Anti-Surb7 antibody (ab123052)
Anti-Surb7 antibody (ab123052) at 1 µg/ml + Mouse liver lysate at 10 µg
Predicted band size : 15 kDa Gel concentration: 10-20%