製品の概要

  • 製品名Anti-SUPT16H antibody
    SUPT16H 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to SUPT16H
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow, Pig
  • 免疫原

    Synthetic peptide:

    DLTNKEGKKPEEK

    with a Cysteine residue linker, corresponding to internal sequence amino acids 385-397 of Human SUPT16H

  • ポジティブ・コントロール
    • HeLa cell lysate Human kidney

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab111934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 120 kDa.
ELISA 1/8000.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the peptidase M24 family. SPT16 subfamily.
  • ドメインThe Glu-rich acidic region in C-terminus is essential for FACT activity.
  • 翻訳後修飾ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.
  • 細胞内局在Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CDC68 antibody
    • Chromatin specific transcription elongation factor 140 kDa subunit antibody
    • Chromatin-specific transcription elongation factor 140 kDa subunit antibody
    • Facilitates chromatin transcription complex subunit SPT16 antibody
    • FACT 140 kDa subunit antibody
    • FACT antibody
    • FACT complex subunit SPT16 antibody
    • FACTp140 antibody
    • FLJ10857 antibody
    • FLJ14010 antibody
    • hSPT16 antibody
    • SP16H_HUMAN antibody
    • Suppressor of Ty 16 homolog antibody
    • Supt16h antibody
    see all

Anti-SUPT16H antibody 画像

  • ab111934, at 3.75 µg/ml, staining SUPT16H in formalin fixed, paraffin embedded Human kidney by Immunohistochemistry.
  • Anti-SUPT16H antibody (ab111934) at 0.1 µg/ml + HeLa cell lysate in RIPA buffer at 35 µg

    Predicted band size : 120 kDa

Anti-SUPT16H antibody (ab111934) 使用論文

ab111934 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab111934.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"